{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:49:32Z","timestamp":1760060972514,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T00:00:00Z","timestamp":1599436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Clindamycin is used for infections caused by Gram\u2010positive and Gram\u2010negative anaerobic pathogens and Gram\u2010positive aerobes. <jats:styled-content style=\"fixed-case\"><jats:italic>Propionibacterium acnes<\/jats:italic><\/jats:styled-content> is an important opportunistic microorganism of the human skin and is related to prostatitis. An LC\u2013electrospray ionization\u2013quadrupole time\u2010of\u2010flight\u2013MS method was validated for determining clindamycin concentrations in plasma and prostate microdialysate. Clindamycin separation was carried out on a C<jats:sub>18<\/jats:sub> column at 0.5\u2009mL\/min. The mobile phase employed gradient elution of formic acid and methanol. A mass spectrometer was operated in positive electrospray ionization mode to monitor ion 425.1784 and 253.1152 for clindamycin and cimetidine (internal standard), respectively. Linearity was obtained at 0.5\u201310.0\u2009\u03bcg\/mL (plasma) and 0.05\u20131.0\u2009\u03bcg\/mL (microdialysate) with coefficients of determination \u22650.999. The intra\u2010 and inter\u2010day precision (coefficient of variation \u2010 CV%) values were \u226413.83% and 12.51% for plasma, respectively, and \u226410.90% and 9.35% for microdialysate, respectively. The accuracy was between 90.82% and 108.25% for plasma, and 96.97% and 106.98% for microdialysate. The present method was fully validated and applied to investigate clindamycin concentrations in both plasma and prostate by microdialysis in Wistar rats (80\u2009mg\/kg, intravenous). Because the penetration of antibiotics into the prostate may be restricted, this method allows us to investigate the prostate concentrations of clindamycin for the first time.<\/jats:p>","DOI":"10.1002\/bmc.4977","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T14:44:53Z","timestamp":1599057893000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Application of an LC\u2013ESI\u2013QTOF\u2013MS method for evaluating clindamycin concentrations in plasma and prostate microdialysate of rats"],"prefix":"10.1002","volume":"34","author":[{"given":"Lisiani","family":"Ritter","sequence":"first","affiliation":[{"name":"Institute of Biotechnology University of Caxias do Sul  Caxias do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduarda","family":"Possa","sequence":"additional","affiliation":[{"name":"College of Pharmacy University of Caxias do Sul  Caxias do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa","family":"Bergoza","sequence":"additional","affiliation":[{"name":"College of Pharmacy University of Caxias do Sul  Caxias do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiana","family":"Agostini","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology University of Caxias do Sul  Caxias do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sidnei M.","family":"Silva","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology University of Caxias do Sul  Caxias do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7102-9204","authenticated-orcid":false,"given":"Leandro","family":"Tasso","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology University of Caxias do Sul  Caxias do Sul Brazil"},{"name":"College of Pharmacy University of Caxias do Sul  Caxias do Sul Brazil"},{"name":"Health Sciences Postgraduate Program University of Caxias do Sul  Caxias do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,7]]},"reference":[{"issue":"7","key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"469","DOI":"10.5414\/CP201478","article-title":"Solid state NMR and bioequivalence comparison of the pharmacokinetic parameters of two formulations of clindamycin","volume":"49","author":"Al\u2010Talla Z. 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