{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:56:14Z","timestamp":1785430574092,"version":"3.56.0"},"reference-count":16,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,4,12]],"date-time":"2005-04-12T00:00:00Z","timestamp":1113264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple, rapid, sensitive and specific liquid chromatography\u2013tandem mass spectrometry method was developed and validated for quantification of tamsulosin (I), a highly selective <jats:italic>\u03b1<\/jats:italic>1\u2010adrenoceptor antagonist used for the treatment of patients with symptomatic benign prostatic hyperplasia. The analyte and internal standard, mosapride (II) were extracted by liquid\u2013liquid extraction with diethyl ether\u2010dichloromethane (70:30, v[sol ]v) using a Glas\u2010Col Multi\u2010Pulse Vortexer. The chromatographic separation was performed on a reverse phase Waters symmetry C<jats:sub>18<\/jats:sub> column with a mobile phase of 0.03% formic acid\u2013acetonitrile (30:70, v[sol ]v). The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The mass transitions <jats:italic>m[sol ]z<\/jats:italic> 409.1 \u2298 228.1 and <jats:italic>m[sol ]z<\/jats:italic> 422.3 \u2298 198.3 were used to measure I and II, respectively. The assay exhibited a linear dynamic range of 0.1\u201350.0 ng[sol ]mL for tamsulosin in human plasma. The lower limit of quantitation was 100 pg[sol ]mL with a relative standard deviation of less than 10%. Acceptable precision and accuracy were obtained for concentrations over the standard curve ranges. A run time of 2.0 min for each sample made it possible to analyze a throughput of more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.498","type":"journal-article","created":{"date-parts":[[2005,4,12]],"date-time":"2005-04-12T14:04:28Z","timestamp":1113314668000},"page":"709-719","source":"Crossref","is-referenced-by-count":28,"title":["Rapid, simple and highly sensitive LC\u2010ESI\u2010MS\/MS method for the quantification of tamsulosin in human plasma"],"prefix":"10.1002","volume":"19","author":[{"given":"N. V. S.","family":"Ramakrishna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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