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A sensitive, rapid method with high recovery for Fosinopril diacid from human plasma was developed. Solid\u2010phase extraction technique employing Waters Oasis SPE cartridges gave clean samples with very high recovery of 97%. The analyte along with its internal standard (Benazepril hydrochloride) were chromatographed on an XTerra RP<jats:sub>8<\/jats:sub> column (4.6\u2009\u00d7\u200950\u2009mm, 5\u2009\u03bcm) using methanol\u2013ammonium acetate buffer (10\u2009m<jats:sc>m;<\/jats:sc> 90:10, v\/v) as the mobile phase. A triple quadrupole mass spectrometer equipped with electrospray ionization source operated in the negative ion mode was used for detection. Multiple reaction monitoring scan mode was used for monitoring the transitions from <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 434.00\u2009\u2192\u2009237.15 for Fosinopril diacid and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 423.10\u2009\u2192\u2009174.00 for Benazepril hydrochloride. Beer\u2013Lambert\u2019s law was obeyed in the range of 0.50\u20131,500.00\u2009ng\/ml (<jats:italic>r<\/jats:italic>\u2009=\u20090.9993). The stability of the drugs in human plasma and in stock solution was proved by performing stability tests as per US Food and Drug Administration guidelines. The method was successfully applied for a bioequivalence study of Fosinopril diacid in 36 healthy, adult, male volunteers under fasting conditions.<\/jats:p>","DOI":"10.1002\/bmc.5047","type":"journal-article","created":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T12:05:18Z","timestamp":1608725118000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A sensitive and efficient LC\u2013MS\/MS method for the bioanalysis of fosinopril diacid from human plasma and its application for a bioequivalence study in humans"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9697-4314","authenticated-orcid":false,"given":"Swati D.","family":"Bhende","sequence":"first","affiliation":[{"name":"Jawaharlal Nehru Technological University Hyderabad  Hyderabad Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8421-7439","authenticated-orcid":false,"given":"Murali Balaram","family":"Varanasi","sequence":"additional","affiliation":[{"name":"Sultan Ul Uloom college of Pharmacy  Hyderabad Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3932-4315","authenticated-orcid":false,"given":"Konde","family":"Abbulu","sequence":"additional","affiliation":[{"name":"CMR college of Pharmacy  Hyderabad Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.04.019"},{"key":"e_1_2_6_3_1","unstructured":"Dolan J. 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