{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:23:45Z","timestamp":1792092225239,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T00:00:00Z","timestamp":1608595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A robust and rapid UPLC\u2013MS\/MS method has been developed, optimized and validated for determination of amlodipine (AML), indapamide (IND) and perindopril (PRN) in human plasma. A positive electrospray ionization mode was used in a Xevo TQD LC\u2013MS\/MS instrument. A single sample preparation step using extraction technique was applied to extract the three analytes from plasma samples. There was no need to extract indapamide from blood samples in a further step. Extraction of the three drugs and internal standards was done using a solvent mixture composed of methyl tertiary butyl ether, dichloromethane and ethyl acetate. The prepared samples were analyzed using an Acquity UPLC HSS C<jats:sub>18<\/jats:sub> (100\u2009\u00d7\u20092.1\u2009mm, 1.7\u2009\u03bcm) column. Ammonium acetate and methanol, pumped at a flow rate of 0.3\u2009ml\/min, were used as a mobile phase. Method validation was done as per the US Food and Drug Administration guidelines. Linearity was achieved in the range of 0.2\u201315\u2009ng\/ml for AML, 0.5\u201350\u2009ng\/ml for IND and 0.5\u2013120\u2009ng\/ml for PRN. Accuracy and precision were estimated and found to be within the acceptable ranges. The rapid chromatography permits analysis of many samples per batch, making the method suitable for clinical and pharmacokinetic investigations. The developed and validated method was applied to estimate AML, IND, and PRN in a fasting bioequivalence study in healthy human volunteers.<\/jats:p>","DOI":"10.1002\/bmc.5048","type":"journal-article","created":{"date-parts":[[2020,12,12]],"date-time":"2020-12-12T02:36:30Z","timestamp":1607740590000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Determination of amlodipine, indapamide and perindopril in human plasma by a novel LC\u2013MS\/MS method: Application to a bioequivalence study"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4425-4390","authenticated-orcid":false,"given":"Mamdouh R.","family":"Rezk","sequence":"first","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamal A.","family":"Badr","sequence":"additional","affiliation":[{"name":"Pharmaceutics Department, Faculty of Pharmacy Deraya University  Egypt"},{"name":"Advanced Research Center, Nasr City  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-8703(89)90834-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(89)90956-9"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1185\/03007997709110219"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-8703(83)90119-9"},{"issue":"1","key":"e_1_2_6_6_1","first-page":"51","article-title":"Determination of amlodipine in human plasma by liquid chromatography\u2013tandem mass spectrometry","volume":"36","author":"Chen X. Y.","year":"2001","journal-title":"Acta Pharmaceutica Sinica"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200666020-00010"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(03)00574-9"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2006.03.039"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.5740\/jaoacint.16-0279"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-005-0548-1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200161060-00020"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.04.008"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.3906\/kim-1712-9"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40119-017-0085-7"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.202000010"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3148"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.68.7830"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4224"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018898624643"},{"issue":"10","key":"e_1_2_6_21_1","first-page":"565","article-title":"Fixed dose combination perindopril\u2013indapamide\u2013amlodipine (Triplixam) for the treatment of arterial hypertension","volume":"69","author":"Scheen A. J.","year":"2014","journal-title":"Revue M\u00e9dicale de Li\u00e8ge"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2000.tb00402.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2017.06.001"},{"issue":"4","key":"e_1_2_6_24_1","first-page":"455","article-title":"Determination of amlodipine in human plasma by electrospray ionization LC\u2013MS\/MS method: Validation and its stability studies","volume":"30","author":"Sirikatitham A.","year":"2008","journal-title":"Songklanakarin Journal of Science and Technology"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-022X(02)00133-1"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2018.05.004"},{"key":"e_1_2_6_27_1","unstructured":"US\u2010FDA. (2018).Bioanalytical Method Validation:Guidance for Industry.https:\/\/www.fda.gov\/files\/drugs\/published\/Bioanalytical-Method-Validation-Guidance-for-Industry.pdf"},{"issue":"10","key":"e_1_2_6_28_1","first-page":"1708","article-title":"Simultaneous determination of telmisartan and amlodipine in dog plasma by LC\u2013MS\u2013MS","volume":"53","author":"Wang B.","year":"2015","journal-title":"Journal of Chromatographic Science"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.05.037"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.08.034"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.01.001"},{"issue":"21","key":"e_1_2_6_32_1","first-page":"1663","article-title":"Determination of amlodipine in human plasma by LC\u2013ESI\u2013MS\/MS method","volume":"41","author":"Zheng F.","year":"2006","journal-title":"Chinese Pharmaceutical Journal"},{"issue":"8","key":"e_1_2_6_33_1","first-page":"383","article-title":"Liquid chromatography\u2013mass spectrometry method for the determination of amlodipine in human plasma and its application in a bioequivalence study","volume":"59","author":"Zou Q.","year":"2009","journal-title":"Arzneimittel\u2010Forschung\/Drug Research"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5048","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.5048","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5048","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:44:38Z","timestamp":1760042678000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5048"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,22]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1002\/bmc.5048"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5048","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,22]]},"assertion":[{"value":"2020-09-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5048"}}