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EDA and EE were separated by reversed\u2010phase HPLC using Agilent ZORBAX SB\u2010Phenyl column, 4.6\u2009mm\u2009\u00d7\u200915\u2009cm, 5\u2009\u03bcm, using a gradient mixture of acetonitrile and Milli\u2010Q water as mobile phase. The linearity and recovery were found in the range of 0.025\u20130.25\u2009mg\/mL and 0.05\u20130.18\u2009mg\/mL for EDA and 0.001\u20130.01\u2009mg\/mL and 0.002\u20130.007\u2009mg\/mL for EE, respectively. The method is validated according to the regulatory guidelines concerning system suitability, specificity, repeatability, recovery, linearity, robustness, and stability of the sample solution.<\/jats:p>","DOI":"10.1002\/bmc.5055","type":"journal-article","created":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T11:37:11Z","timestamp":1608809831000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Novel reversed\u2010phase HPLC method for simultaneous determination of ethynodiol diacetate (EDA)\/ethinyl estradiol (EE) in pharmaceutical dosage form"],"prefix":"10.1002","volume":"35","author":[{"given":"Srinivasa Reddy","family":"Chinta","sequence":"first","affiliation":[{"name":"Department of Quality Operations Novast Laboratories Ltd  Nantong Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijetha","family":"Ponnam","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Vignan\u2019s Foundation for Science Technology and Research University  Guntur Andhra Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5737-8528","authenticated-orcid":false,"given":"Naresh Kumar","family":"Katari","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Science GITAM Deemed to be University  Hyderabad Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sasikiran Goud","family":"Ediga","sequence":"additional","affiliation":[{"name":"Department of Quality Operations Novast Laboratories Ltd  Nantong Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishnu Murthy","family":"Marisetti","sequence":"additional","affiliation":[{"name":"Analytical Research and Development ScieGen Pharmaceuticals, Inc.  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