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Analytes were extracted from 50\u2009\u03bcL human whole blood by protein precipitation. The separation of the drugs was performed on an Acquity UPLC BEH C18 column. Analytes were eluted with a mobile phase consisting of 2\u2009mM ammonium acetate with 0.1% formic acid (v\/v) in deionised water and 2 mM ammonium acetate with 0.1% formic acid (v\/v) in methanol at a flow rate of 300\u2009\u03bcL\/min in gradient elution. The method performance was evaluated by analysing patient blood samples and\/or external quality control samples [proficiency testing (PT) scheme]. The method was linear from 23.75 to 1094.0, 1.3 to 42.4, 1.3 to 47.0 and 1.2\u201341.6 \u03bcg\/mL for CYC, TAC, SIR and EVE, respectively. The within\u2010 and between\u2010assay reproducibility results were \u02c2 11%. Results from PT and patient sample quantification were comparable to those obtained previously by an in\u2010house validated method using protein precipitation and liquid\u2013liquid extraction. This method showed good analytical performance for quantifying CYC, TAC, SIR and EVE in whole blood over their respective calibration ranges.<\/jats:p>","DOI":"10.1002\/bmc.5071","type":"journal-article","created":{"date-parts":[[2021,1,19]],"date-time":"2021-01-19T03:10:10Z","timestamp":1611025810000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Simultaneous quantification of cyclosporin, tacrolimus, sirolimus and everolimus in whole blood by UHPLC\u2013MS\/MS for therapeutic drug monitoring"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7820-552X","authenticated-orcid":false,"given":"Natalicia J.","family":"Antunes","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Faculty of Medical Sciences State University of Campinas (UNICAMP)  Campinas SP Brazil"},{"name":"Clinical Pharmacology, William Harvey Research Institute, Barts and The London School of Medicine and Dentistry Queen Mary University of London  London UK"},{"name":"Analytical Services International (ASI) Ltd St George\u2019s\u2014University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Kipper","sequence":"additional","affiliation":[{"name":"Analytical Services International (ASI) Ltd St George\u2019s\u2014University of London  London UK"},{"name":"Institute of Chemistry University of Tartu  Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewis","family":"Couchman","sequence":"additional","affiliation":[{"name":"Analytical Services International (ASI) Ltd St George\u2019s\u2014University of London  London UK"},{"name":"Pharmaceutical Sciences Clinical Academic Group King\u2019s College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Anne","family":"Duncan","sequence":"additional","affiliation":[{"name":"Analytical Services International (ASI) Ltd St George\u2019s\u2014University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Holt","sequence":"additional","affiliation":[{"name":"Analytical Services International (ASI) Ltd St George\u2019s\u2014University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto","family":"De Nucci","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medical Sciences State University of Campinas (UNICAMP)  Campinas SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atholl","family":"Johnston","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology, William Harvey Research Institute, Barts and The London School of Medicine and Dentistry Queen Mary University of London  London UK"},{"name":"Analytical Services International (ASI) Ltd St George\u2019s\u2014University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.21958"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2016.39"},{"key":"e_1_2_5_4_1","unstructured":"EMA. 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