{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T12:45:02Z","timestamp":1761741902926,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,6,27]],"date-time":"2001-06-27T00:00:00Z","timestamp":993600000000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2001,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Three methods for the determination of rat hepatocellular thiols by high\u2010performance liquid chromatography (HPLC) with fluorescence (FL) detection have been developed. The thiols in the cells were tagged with three fluorogenic reagents, SBD\u2010F, ABD\u2010F and DBD\u2010F. These reagents could permeate into cells and effectively reacted with thiols to produce highly fluorescent derivatives. These derivatives fluoresced in the long wavelength region at around 530\u2005nm (excitation at around 380 nm). The five biological thiols tagged were perfectly separated by reversed\u2010phase liquid chromatography and were sensitively and selectively detected without any interference from endogenous substanaces. The main thiol in the cells was reduced GSH and the concentration was at the\u2005m<jats:sc>M<\/jats:sc> level. The proposed procedures were applied to the determination of hepatocellular GSH after treatment of environmental pollutants such as volatile organic compounds (VOC) and endocrine disrupting chemicals (EDC). From the comparison of intracellular GSH concentration, the test compounds were classified into four groups: compounds of strong depletion (eg triphenyltin chloride, hexachlorocyclohexene, nonylphenol, bromoacetic acid, 4\u2010chlorobenzyl chloride and 1,3\u2010dichloropropene), slight decrease (eg bisphenol A, benzo[<jats:italic>a<\/jats:italic>]pylene, carbon tetrachloride and benzene), slight increase (eg bromoform and toluene), and no effect (eg 1,1,1\u2010trichloroethane, 1,1,2\u2010trichloroethane and 1,2\u2010dichloroethane). Although the decrease of GSH concentration does not reflect the cytotoxicity of chemicals, the proposed procedure utilizing isolated rat hepatpcytes seems to be useful for investigating the bioactivation of VOC, and EDC, etc. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.51","type":"journal-article","created":{"date-parts":[[2002,9,10]],"date-time":"2002-09-10T21:23:48Z","timestamp":1031693028000},"page":"240-247","source":"Crossref","is-referenced-by-count":15,"title":["Determination of rat hepatocellular glutathione by reversed\u2010phase liquid chromatography with fluorescence detection and cytotoxicity evaluation of environmental pollutants based on the concentration change"],"prefix":"10.1002","volume":"15","author":[{"given":"Toshimasa","family":"Toyo'oka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junko","family":"Tanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideto","family":"Jinno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,6,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(79)90161-3"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00736807"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(81)90224-1"},{"key":"e_1_2_1_5_1","first-page":"225","author":"Giger W","year":"1984","journal-title":"Science"},{"issue":"2","key":"e_1_2_1_6_1","first-page":"511","volume":"106","author":"Guillouzo A","year":"1998","journal-title":"Environmental Health Perspectives"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(81)90488-3"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1515\/REVEH.1996.11.4.213"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(00)84095-7"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-2333(97)00033-7"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90438-6"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.132.6.8504731"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/03602537908997468"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6836290"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.52.070183.003431"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00119a010"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287397909529766"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(82)90143-0"},{"volume-title":"Modern Derivatization Methods for Separation Sciences","year":"1999","author":"Toyo'oka T","key":"e_1_2_1_19_1"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)91626-1"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/an9840901003"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00277a044"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00286a032"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/an9891400413"},{"key":"e_1_2_1_25_1","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1016\/S0026-895X(25)13561-X","volume":"14","author":"Tunek A","year":"1978","journal-title":"Molecular Pharmacology"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-011-3356-2_26"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0773.1983.tb02686.x"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0048-9697(99)00226-0"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.51","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.51","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:56:42Z","timestamp":1760043402000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.51"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,6]]},"references-count":27,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,6]]}},"alternative-id":["10.1002\/bmc.51"],"URL":"https:\/\/doi.org\/10.1002\/bmc.51","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2001,6]]}}}