{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T18:55:43Z","timestamp":1773082543675,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,4,26]],"date-time":"2005-04-26T00:00:00Z","timestamp":1114473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple, rapid, sensitive and specific liquid chromatography\u2013tandem mass spectrometry method was developed and validated for quantification of hydrochlorothiazide (I), a common diuretic and anti\u2010hypertensive agent. The analyte and internal standard, tamsulosin (II) were extracted by liquid\u2013liquid extraction with diethyl ether\u2013dichloromethane (70:30, v[sol ]v) using a Glas\u2010Col Multi\u2010Pulse Vortexer. The chromatographic separation was performed on a reversed\u2010phase column (Waters symmetry C<jats:sub>18<\/jats:sub>) with a mobile phase of 10 mm ammonium acetate\u2013methanol (15:85, v[sol ]v). The protonated analyte was quantitated in negative ionization by multiple reaction monitoring with a mass spectrometer. The mass transitions <jats:italic>m[sol ]z<\/jats:italic> 296.1 \u2298 205.0 and <jats:italic>m[sol ]z<\/jats:italic> 407.2 \u2298 184.9 were used to measure I and II, respectively. The assay exhibited a linear dynamic range of 0.5\u2013200 ng[sol ]mL for hydrochlorothiazide in human plasma. The lower limit of quantitation was 500 pg[sol ]mL, with a relative standard deviation of less than 9%. Acceptable precision and accuracy were obtained for concentrations over the standard curve ranges. A run time of 2.5 min for each sample made it possible to analyze a throughput of more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.510","type":"journal-article","created":{"date-parts":[[2005,4,26]],"date-time":"2005-04-26T13:48:19Z","timestamp":1114523299000},"page":"751-760","source":"Crossref","is-referenced-by-count":41,"title":["Sensitive liquid chromatography\u2013tandem mass spectrometry method for quantification of hydrochlorothiazide in human plasma"],"prefix":"10.1002","volume":"19","author":[{"given":"N. V. S.","family":"Ramakrishna","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. 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