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The first technique was UPLC; the separation and resolution of both analytes were achieved using a Zorbax eclipse plus C<jats:sub>18<\/jats:sub> column applying an isocratic elution based on phosphate buffer pH 4\u2013acetonitrile (65:35, v\/v) as a running mobile phase at flow rate 1.5\u00a0ml\/min and the effluent was monitored at 220\u2009nm. Augmentation of Lean Six Sigma with UPLC and HPTLC methods had a major impact on the development of robust specifications to ensure that the quality at six sigma level has a high level of statistical confidence and target performance. On the chromatogram, empagliflozin and linagliptin appeared at retention times of 1.417 and 2.453\u2009min, respectively. The second technique was HPTLC; both analytes were fairly well resolved and separated using a developing mobile phase composed of ethyl acetate\u2013chloroform\u2013acetonitrile (55:25:20 by volume). The values of retention factor (R<jats:sub>F<\/jats:sub>) were 0.29 and 0.53 for linagliptin and empagliflozin, respectively. All variables were investigated to adjust the whole conditions.<\/jats:p>","DOI":"10.1002\/bmc.5102","type":"journal-article","created":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T03:38:34Z","timestamp":1614224314000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Determination of linagliptin and empagliflozin by UPLC and HPTLC techniques aided by lean six sigma approach"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0820-4449","authenticated-orcid":false,"given":"Ebrahim A.","family":"El\u2010Desouky","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Al\u2010Azhar University  Nasr City Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4111-9716","authenticated-orcid":false,"given":"Ahmed M.","family":"Abdel\u2010Raoof","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Al\u2010Azhar University  Nasr City Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf","family":"Abdel\u2010Fattah","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Al\u2010Azhar University  Nasr City Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Abdel\u2010Zaher","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Al\u2010Azhar University  Nasr City Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman O. 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