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The current study aimed to evaluate the effects of <jats:italic>F. spragueana<\/jats:italic> leaf extract on nephrotoxicity caused by gentamicin. Gentamicin is an important broad\u2010spectrum antibiotic; nevertheless, it exhibits serious nephrotoxic adverse effects. HPLC\u2013ESI\/MS spectrometric analysis of the extract revealed the presence of 37 phenolic compounds. Moreover, five compounds were isolated from the leaf extract, and identified on the basis of spectroscopic analysis. The isolated compounds were syringic acid (<jats:bold>1<\/jats:bold>), <jats:italic>p\u2010<\/jats:italic>coumaric acid (<jats:bold>2<\/jats:bold>), 3\u2032,5\u2032 <jats:italic>O\u2010<\/jats:italic>dicaffeoylquinic acid (<jats:bold>3<\/jats:bold>), luteolin\u20108\u2010C\u2010\u03b2\u2010D glucopyranoside (orientin) (<jats:bold>4<\/jats:bold>) and 8\u2010methoxy kaempferol\u20103\u2010<jats:italic>O\u2010<\/jats:italic>[<jats:italic>\u03b1<\/jats:italic>\u2010L\u2010rhamnopyranosyl (1\u21922) \u03b2\u2010D\u2010glucopyranoside] (<jats:bold>5<\/jats:bold>). The gentamicin\u2010induced nephrotoxicity model was used to evaluate the protective effect of <jats:italic>F. spragueana<\/jats:italic> on renal toxicity biomarkers throughout the development of acute kidney injury. Administration of extract led to improvement in kidney function through inhibition of kidney injury molecule\u20101, creatinine, blood urea nitrogen and total bilirubin, as well as decreasing the inflammatory markers interlukin1\u2010beta and myeloperoxidase. Furthermore, it reduced the oxidative stress by increasing reduced glutathione and total antioxidant capacity levels while decreasing malondialdehyde and nitric oxide content, and improved renal histopathological injuries.<\/jats:p>","DOI":"10.1002\/bmc.5135","type":"journal-article","created":{"date-parts":[[2021,4,5]],"date-time":"2021-04-05T08:06:53Z","timestamp":1617610013000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["HPLC\u2013ESI\/MS profiling, phytoconstituent isolation and evaluation of renal function, oxidative stress and inflammation in gentamicin\u2010induced nephrotoxicity in rats of <i>Ficus spragueana<\/i> Mildbr. &amp; Burret"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9321-1971","authenticated-orcid":false,"given":"Rehab F.","family":"Taher","sequence":"first","affiliation":[{"name":"Natural Compounds Chemistry Department, Pharmaceutical and Drug Industries Research Division National Research Centre (ID 60014618)  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6165-1873","authenticated-orcid":false,"given":"Mona A.","family":"Raslan","sequence":"additional","affiliation":[{"name":"Pharmacognosy Department, Pharmaceutical and Drug Industries Research Division National Research Centre (ID 60014618)  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0141-526X","authenticated-orcid":false,"given":"Marwa A.","family":"Masoud","sequence":"additional","affiliation":[{"name":"Pharmacology Department, National Organization for Drug Control and Research Egyptian Drug Authority  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2493-4663","authenticated-orcid":false,"given":"Mahmoud I.","family":"Nassar","sequence":"additional","affiliation":[{"name":"Natural Compounds Chemistry Department, Pharmaceutical and Drug Industries Research Division National Research Centre (ID 60014618)  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9366-6431","authenticated-orcid":false,"given":"Mona E.","family":"Aboutabl","sequence":"additional","affiliation":[{"name":"Medicinal and Pharmaceutical Chemistry Department (Pharmacology Group), Pharmaceutical and Drug Industries Research Division National Research Centre (ID 60014618)  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4,22]]},"reference":[{"key":"e_1_2_7_2_1","article-title":"Anti\u2010eczematic activity and flavonoid constituents from the leaves of Ficus Spragueana Mildbr. and Burret","author":"Abbass H.","year":"2011","journal-title":"Al\u2010Azhar Journal of Pharmaceutical Sciences, 2008"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.04.022"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.7324\/JAPS.2018.8714"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327116689716"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.5958\/0974-360X.2017.00313.4"},{"key":"e_1_2_7_7_1","first-page":"37","article-title":"Bisheri Booti (Aerva lanata): Some lesser\u2010known uses and pharmacognosy","volume":"5","author":"Afaq S. 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