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Samples were extracted with chloroform and separation was achieved with a Symmetry RP<jats:sub>18<\/jats:sub> column. Ultraviolet absorption was measured at 254\u2009nm. A combination of 0.1% formic acid and acetonitrile (50:50) was used as the mobile phase. The calibration curve was linear from 0.1\u201325\u2009\u03bcg\/mL, with a lower limit of quantification of 0.1\u2009\u03bcg\/mL with a 100\u2009\u03bcL sample. The precision and accuracy were well within the range set by the Food and Drug Administration and the recovery was over 95%. This technique was used to analyze ponazuril samples and found to be appropriate for pharmacokinetic studies.<\/jats:p>","DOI":"10.1002\/bmc.5210","type":"journal-article","created":{"date-parts":[[2021,7,3]],"date-time":"2021-07-03T04:11:24Z","timestamp":1625285484000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bioanalytical RP\u2010HPLC method for the determination of ponazuril in plasma"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5184-900X","authenticated-orcid":false,"given":"Sherry","family":"Cox","sequence":"first","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences College of Veterinary Medicine, The University of Tennessee  Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lainey","family":"Harvill","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences College of Veterinary Medicine, The University of Tennessee  Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Singleton","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences College of Veterinary Medicine, The University of Tennessee  Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0896-6681","authenticated-orcid":false,"given":"Joan","family":"Bergman","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences College of Veterinary Medicine, The University of Tennessee  Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Becky","family":"DeBolt","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine, The University of Tennessee  Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2008.01053.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2011.01315.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2008.01039.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-005-1456-x"},{"key":"e_1_2_7_6_1","unstructured":"FDA\/Center for Drug Evaluation and Research (CDER) Reviewer Guidance: Validation of Chromatographic Methods. 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