{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T23:47:25Z","timestamp":1788824845665,"version":"build-2803163510"},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T00:00:00Z","timestamp":1628467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Remdesivir (RDV) is the first antiviral drug, approved by the Food and Drug Administration, to treat severe acute respiratory syndrome coronavirus 2. RDV is a relatively new chemical entity, \u2018ester prodrug\u2019, with no reported stability profile. Due to the urgency of its use and thus fast production, it is important to develop a stability\u2010indicating method for its assay. Chromatographic separation was carried out on a C18 column (250\u2009\u00d7\u20094.6\u2009mm, 5 \u00b5m) with dual detection: diode array at 240\u2009nm and fluorescence at <jats:italic>\u03bb<\/jats:italic><jats:sub>ex\/em<\/jats:sub> 245\/390\u2009nm. Isocratic elution of acetonitrile and distilled water (acidified with phosphoric acid, pH\u20094) in the ratio of 55:45 (v\/v), respectively, was used. The linearity range using HPLC\u2010diode array detection was 0.1\u201315\u2009\u03bcg\/mL, whereas that using fluorimetric detection was 0.05\u201315\u2009\u03bcg\/mL. As per the International Conference on Harmonization guidelines, RDV has been degraded by accelerated alkaline, acidic, neutral hydrolysis, oxidative, heat, and photolytic stress conditions. Possible degradation hypothesis of the parent molecule has been suggested and illustrated. The proposed methods have achieved selective determination of the intact drug with no peaks overlapping in all assumptions. Extensive degradation confirms threatened drug stability at thermal and basic hydrolytic stressing. The developed methods were fully validated and proved suitable for quality control routine analysis of RDV in raw material and pharmaceutical dosage forms.<\/jats:p>","DOI":"10.1002\/bmc.5212","type":"journal-article","created":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T21:05:56Z","timestamp":1625519156000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Accelerated stability study of the ester prodrug remdesivir: Recently FDA\u2010approved Covid\u201019 antiviral using reversed\u2010phase\u2010HPLC with fluorimetric and diode array detection"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7119-2754","authenticated-orcid":false,"given":"Mohamed M. A.","family":"Hamdy","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Pharos University in Alexandria  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8856-7949","authenticated-orcid":false,"given":"Mona M.","family":"Abdel Moneim","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Pharos University in Alexandria  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8940-6370","authenticated-orcid":false,"given":"Miranda F.","family":"Kamal","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Damanhour University  Beheira Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2020-0612"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa152"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.22270\/jddt.v10i4-s.4313"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2020.e04561","article-title":"Stability\u2010indicating UPLC method for the determination of curcumin diethyl disuccinate, an ester prodrug of curcumin, in raw materials","volume":"6","author":"Bhuket P. 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(2020).FDA Approves First Treatment for COVID\u201019.https:\/\/www.fda.gov\/news-events\/press-announcements\/fda-approves-first-treatmentcovid-19. (Accessed Jan 19 2021).","DOI":"10.1038\/d41573-020-00025-z"},{"key":"e_1_2_6_20_1","unstructured":"United States Pharmacopeial Convention USP 42 \u2010 NF 37 the United States pharmacopeia and national formulary(2019) USA."},{"key":"e_1_2_6_21_1","first-page":"617","article-title":"Validation of LC\u2010MS\/MS methods for determination of remdesivir and its metabolites GS\u2010441524 and GS\u2010704277 in acidified human plasma and their application in COVID\u201019 related clinical studies","volume":"15","author":"Xiao D.","year":"2021","journal-title":"Analytical Biochemistry"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5212","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.5212","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5212","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:14:03Z","timestamp":1760044443000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,9]]},"references-count":20,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/bmc.5212"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5212","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,9]]},"assertion":[{"value":"2021-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5212"}}