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This assay can be combined with our method for osimertinib, allowing quantification of the most used <jats:italic>ALK<\/jats:italic>\u2010 and <jats:italic>EGFR<\/jats:italic>\u2010tyrosine kinase inhibitors (TKIs) in non\u2010small cell lung cancer with a single\u2010assay setup. Chromatographic separation was performed on a HyPurity\u00ae C<jats:sub>18<\/jats:sub> analytical column using an elution gradient of ammonium acetate in water and in methanol, both acidified with formic acid 0.1%. Detection and quantification were performed using a triple quad mass spectrometer with an electrospray ionization interface. This method led to robust results, as the selectivity, carryover, precision and accuracy met all pre\u2010specified requirements. The assay was validated over a linear range of 100\u20132,000\u2009ng\/ml for alectinib and erlotinib and 50\u20131,000\u2009ng\/ml for crizotinib and gefitinib. Alectinib, crizotinib, erlotinib and gefitinib were all stable for at least 4\u00a0h in whole blood (at room temperature and at 4\u00b0C) and for at least 1 month in EDTA plasma when stored at \u221280\u00b0C, while osimertinib proved to be unstable at room temperature. Although high\u2010performance liquid chromatography was used, the run time was short and comparable with other methods using ultra\u2010high performance liquid chromatography.<\/jats:p>","DOI":"10.1002\/bmc.5224","type":"journal-article","created":{"date-parts":[[2021,8,7]],"date-time":"2021-08-07T08:30:47Z","timestamp":1628325047000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Development and validation of an HPLC\u2013MS\/MS method to simultaneously quantify alectinib, crizotinib, erlotinib, gefitinib and osimertinib in human plasma samples, using one assay run"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4869-5791","authenticated-orcid":false,"given":"Ard","family":"van Veelen","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacy and Toxicology Maastricht University Medical Center+  Maastricht the Netherlands"},{"name":"CARIM School for Cardiovascular Disease Maastricht University Medical Center+  Maastricht the Netherlands"},{"name":"Department of Pharmacoepidemiology and Clinical Pharmacology Utrecht Institute for Pharmaceutical Sciences  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"van Geel","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Toxicology Maastricht University Medical Center+  Maastricht the Netherlands"},{"name":"CARIM School for Cardiovascular Disease Maastricht University Medical Center+  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy","family":"Schoufs","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Toxicology Maastricht University Medical Center+  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvo","family":"de Beer","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Toxicology Maastricht University Medical Center+  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo M.","family":"Stolk","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Toxicology Maastricht University Medical Center+  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizza E. L.","family":"Hendriks","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Diseases, GROW\u2010School for Oncology and Developmental Biology Maastricht University Medical Center+  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sander","family":"Croes","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Toxicology Maastricht University Medical Center+  Maastricht the Netherlands"},{"name":"CARIM School for Cardiovascular Disease Maastricht University Medical Center+  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1810171"},{"key":"e_1_2_9_3_1","unstructured":"European Medicines Agency. 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