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In this study, arial parts of <jats:styled-content style=\"fixed-case\"><jats:italic>A. racemosus<\/jats:italic><\/jats:styled-content> were investigated for their total phenolic content, total flavonoid content and antioxidative potential. A high\u2010performance thin\u2010layer chromatography (HPTLC) method combined with effect\u2010directed\u2010analysis was also developed to screen the antioxidant effects of <jats:styled-content style=\"fixed-case\"><jats:italic>A. racemosus<\/jats:italic><\/jats:styled-content> and quantify biologically active compounds on chromatograms from <jats:italic><jats:styled-content style=\"fixed-case\">A. racemosus<\/jats:styled-content><\/jats:italic>. Total phenolics (154\u2009mg gallic acid equivalent\/g), flavonoid contents (497\u2009mg quercetin\/g) and IC<jats:sub>50<\/jats:sub> (15.25\u2009\u03bcg\/ml) were found to be higher in methanolic extract of <jats:styled-content style=\"fixed-case\"><jats:italic>A. racemosus<\/jats:italic><\/jats:styled-content> than in <jats:italic>n<\/jats:italic>\u2010hexane, chloroform and ethyl acetate extracts. HPTLC hyphenated with chemical derivatizations (DPPH\u2022, <jats:italic>p<\/jats:italic>\u2010anisaldehyde\/sulfuric acid, and ferric chloride) was used to evaluate antioxidant activity and the presence of phytosterols, terpenoids and polyphenolic contents. The same compounds at 100*retention factor\u2009=\u200958, 68, 74 and 65 in extracts were responsible for antioxidant activity. Hyphenated HPTLC allowed a rapid characterization of the active compound with a combination of effect\u2010directed\u2010analysis and attenuated total reflectance\u2013Fourier transform infrared spectroscopy. Spectral analysis of the band from attenuated total reflectance identified myricetin, quercetin, <jats:italic>p<\/jats:italic>\u2010coumaric acid and caffeic acid as responsible for the antioxidant activity.<\/jats:p>","DOI":"10.1002\/bmc.5230","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T15:46:57Z","timestamp":1629301617000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["High\u2010performance thin\u2010layer chromatography based approach for bioassay and ATR\u2013FTIR spectroscopy for the evaluation of antioxidant compounds from <i>Asparagus racemosus<\/i> Willd. aerial parts"],"prefix":"10.1002","volume":"35","author":[{"given":"Gouhar Jahan","family":"Ashraf","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priya","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarun Kumar","family":"Dua","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paramita","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gouranga","family":"Nandi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2229-1019","authenticated-orcid":false,"given":"Ranabir","family":"Sahu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology University of North Bengal  Darjeeling India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.460970"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/md17030148"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2020.113488"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants8040096"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2018.1560310"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12161-015-0270-z"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2018.00087"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2018.1459173"},{"key":"e_1_2_7_10_1","unstructured":"Guideline I. 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