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Paroxetine hydrochloride was used as the internal standard, and samples were extracted by protein precipitation with acetonitrile. The non\u2010enantioselective method was conducted using a C18 column, and the mobile phase consisted of water for solvent A and acetonitrile for solvent B, both with 0.1% formic acid. For the chiral method, an analytical column Lux Cellulose\u20101 was used. Mobile phase A was composed of water with 0.025% of formic acid and 0.05% of diethylamine, and mobile phase B consisted of acetonitrile:2\u2010propanol (95:5, v\/v). No significant matrix effects were observed at the retention times of analytes and internal standard. The mean recovery was 89%, and the assays were linear in the concentration range of 1\u201350 and 5\u201330\u2009ng\/mL for the non\u2010enantioselective and enantioselective methods, respectively. The intra\u2010 and inter\u2010day precisions of both methods were less than 12.30%, and the accuracies were less than 12.13%. The validated methods were successfully applied to a pharmacokinetic study in which 20\u2010mg CTP tablets were administered to healthy volunteers, and their plasma levels were monitored over time in a bioequivalence study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Highlights<\/jats:title><jats:p>\n<jats:list>\n<jats:list-item><jats:p>Simple and rapid LC\u2013MS\/MS method for the quantification of citalopram and its enantiomers in human plasma.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Both methods were demonstrated to be selective, reliable, and sensitive.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Both methods have sufficient sensitivity to quantify the steady state through concentrations already reported for citalopram and escitalopram.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Validated method presented in this study can be suitably applied to pharmacokinetic studies involving citalopram and escitalopram.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Bland\u2013Altman analysis suggested that non\u2010enantioselective and enantioselective methods can be applied in pharmacokinetic studies.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/bmc.5237","type":"journal-article","created":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T15:17:55Z","timestamp":1630509475000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Selective LC\u2013MS\/MS determination of citalopram enantiomers and application to a pharmacokinetic evaluation of generic and reference formulations"],"prefix":"10.1002","volume":"36","author":[{"given":"Carlos Eduardo Miranda","family":"de Sousa","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences Federal University of Pernambuco  Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noely Camila Tavares Cavalcanti","family":"Bedor","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Federal University of Pernambuco  Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovana Damasceno","family":"Sousa","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Federal University of Pernambuco  Recife Brazil"},{"name":"Center for Biological and Health Sciences Federal University of Western Bahia  Barreiras Bahia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilherme Henrique On\u00f3rio","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Graduate Program in Pharmaceutical Sciences Federal University of S\u00e3o Jo\u00e3o del\u2010Rei  Divin\u00f3polis MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leila Bastos","family":"Leal","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Federal University of Pernambuco  Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3901-9126","authenticated-orcid":false,"given":"Danilo Cesar Galindo","family":"Bedor","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Federal University of Pernambuco  Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8354-1843","authenticated-orcid":false,"given":"Whocely Victor","family":"de Castro","sequence":"additional","affiliation":[{"name":"Graduate Program in Pharmaceutical Sciences Federal University of S\u00e3o Jo\u00e3o del\u2010Rei  Divin\u00f3polis MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davi Pereira","family":"de Santana","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Federal University of Pernambuco  Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,3]]},"reference":[{"volume-title":"Guideline on the investigation of bioequivalence","year":"2010","author":"Agency E. 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