{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:56:01Z","timestamp":1760061361263,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,28]],"date-time":"2021-11-28T00:00:00Z","timestamp":1638057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K23HD101651"],"award-info":[{"award-number":["K23HD101651"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Furosemide is a diuretic drug used to increase urine flow in order to reduce the amount of salt and water in the body. It is commonly utilized to treat preterm infants with chronic lung disease of prematurity. There is a need for a simple and reliable quantitation of furosemide in human urine. We have developed and validated an ultra\u2010high performance liquid chromatography\u2013tandem mass spectrometry method for furosemide quantitation in human urine with an assay range of 0.100\u201350.0\u00a0\u03bcg\/ml. Sample preparation involved solid\u2010phase extraction with 10\u00a0\u03bcl of urine. Intra\u2010day accuracies and precisions for the quality control samples were 94.5\u2013106 and 1.86\u201310.2%, respectively, while inter\u2010day accuracies and precision were 99.2\u2013102 and 3.38\u20137.41%, respectively. Recovery for furosemide had an average of 23.8%, with an average matrix effect of 101%. Furosemide was stable in human urine under the assay conditions. Stability for furosemide was shown at 1\u00a0week (room temperature, 4, \u221220 and \u221278\u00b0C), 6\u00a0months (\u221278\u00b0C), and through three freeze\u2013thaw cycles. This robust assay demonstrates accurate and precise quantitation of furosemide in a small volume (10\u00a0\u03bcl) of human urine. It is currently being implemented in an ongoing pediatric clinical study.<\/jats:p>","DOI":"10.1002\/bmc.5262","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T09:47:29Z","timestamp":1634204849000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development, validation, and implementation of an UHPLC\u2013MS\/MS method for the quantitation of furosemide in infant urine samples"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8310-4715","authenticated-orcid":false,"given":"Christina","family":"Vedar","sequence":"first","affiliation":[{"name":"Center for Clinical Pharmacology Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6427-093X","authenticated-orcid":false,"given":"Nicolas A.","family":"Bamat","sequence":"additional","affiliation":[{"name":"Center for Clinical Pharmacology Children's Hospital of Philadelphia  Philadelphia PA USA"},{"name":"Division of Neonatology Children's Hospital of Philadelphia  Philadelphia PA USA"},{"name":"Center for Pediatric Clinical Effectiveness Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8662-7474","authenticated-orcid":false,"given":"Athena F.","family":"Zuppa","sequence":"additional","affiliation":[{"name":"Center for Clinical Pharmacology Children's Hospital of Philadelphia  Philadelphia PA USA"},{"name":"Department of Anesthesiology and Critical Care Medicine Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan E.","family":"Reilly","sequence":"additional","affiliation":[{"name":"Center for Pediatric Clinical Effectiveness Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8779-6034","authenticated-orcid":false,"given":"Ganesh S.","family":"Moorthy","sequence":"additional","affiliation":[{"name":"Center for Clinical Pharmacology Children's Hospital of Philadelphia  Philadelphia PA USA"},{"name":"Department of Anesthesiology and Critical Care Medicine Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-3476(82)80319-3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-3476(78)81181-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.13505"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41372-019-0415-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2011.09.003"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.02.045"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2012.02699.x"},{"key":"e_1_2_9_9_1","unstructured":"FDA(2018). 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