{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:55:54Z","timestamp":1760061354117,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T00:00:00Z","timestamp":1639958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFC1702006"],"award-info":[{"award-number":["2017YFC1702006"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018617","name":"Liaoning Revitalization Talents Program","doi-asserted-by":"publisher","award":["XLYC1907103"],"award-info":[{"award-number":["XLYC1907103"]}],"id":[{"id":"10.13039\/501100018617","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702995","81573501"],"award-info":[{"award-number":["81702995","81573501"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["DUT21LK11"],"award-info":[{"award-number":["DUT21LK11"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cytochrome P450 46A1 (CYP46A1) is a key enzyme responsible for metabolizing cholesterol to 24\u2010hydroxycholesterol in the brain, and thus might serve as a therapeutic target for several neurodegenerative disorders including Parkinson's disease, Alzheimer's disease and Huntington's disease. However, an applicable, sensitive and reliable method for the precise measurement of CYP46A1 activities in complex biological samples remains limited. In this study, a novel ultra\u2010high\u2010performance liquid chromatography\u2013tandem mass spectrometry (UPLC\u2013MS\/MS) method for highly sensitive and selective determination of 24\u2010hydroxycholesterol was developed to characterize CYP46A1 activity. The mass spectrometric detection was performed using multiple reaction monitoring for 24\u2010hydroxcholesterol at <jats:italic>m\/z<\/jats:italic> 385.2\u2009\u2192\u2009367.2. The limit of quantification for 24\u2010hydroxycholesterol using this UPLC\u2013MS\/MS method was as low as 10\u2009nM, which is lower than those reported previously. The method also showed favorable accuracy and precision. Meanwhile, the short\u2010 and long\u2010term stability of this method was fully validated. In addition, the method was successfully applied to investigate the kinetic properties of 24\u2010hydroxycholesterol formation by CYP46A1.<\/jats:p>","DOI":"10.1002\/bmc.5291","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T23:10:42Z","timestamp":1638400242000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Highly sensitive and selective detection of cytochrome P450 46A1 activity by a ultra\u2010high\u2010performance liquid chromatography\u2013tandem mass spectrometry method"],"prefix":"10.1002","volume":"36","author":[{"given":"Shujuan","family":"Wang","sequence":"first","affiliation":[{"name":"School of Life and Pharmaceutical Sciences Dalian University of Technology  Panjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life and Pharmaceutical Sciences Dalian University of Technology  Panjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life and Pharmaceutical Sciences Dalian University of Technology  Panjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life and Pharmaceutical Sciences Dalian University of Technology  Panjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Life and Pharmaceutical Sciences Dalian University of Technology  Panjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Pharmacy Dalian Medical University  Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Pharmacy Dalian Medical University  Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6173-8186","authenticated-orcid":false,"given":"Yong","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life and Pharmaceutical Sciences Dalian University of Technology  Panjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12868-014-0137-z"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmz021"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/c4an00517a"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2008.12.050"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.05.037"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awv166"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1110"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M051508"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.08.006"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.45.6.1092"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.56.12.1683"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2010.07.049"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.2006.00683.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.794909"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.112.080424"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2017.06.026"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803717105"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2016.09.011"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311-019-00731-6"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21605"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2019.01.011"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.78.072407.103859"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1997.tb48466.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/42.4.531"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.12.013"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2013.07.016"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5291","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.5291","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:51:54Z","timestamp":1760050314000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,20]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1002\/bmc.5291"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5291","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2021,12,20]]},"assertion":[{"value":"2021-09-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5291"}}