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Subsequently, KYN acts as a precursor of some neuroactive metabolites such as kynurenic acid, quinolinic acid, and an important enzyme co\u2010factor, nicotine adenine dinucleotide. These metabolites of tryptophan are a part of the \u2018kynurenine pathway.\u2019 In addition, KYN functions as an endogenous ligand for the aryl hydrocarbon receptor, which acts as a transcription factor. The levels of tryptophan metabolites are important for the assessment of the stage of neurological disorders, and therefore have garnered significant interest for clinical diagnosis. In this review, the detection of kynurenine, kynurenic acid, quinolinic acid, and other tryptophan metabolites performed via chromatographic methods such as HPLC using UV absorbance, fluorescence, and chromatographic\u2010mass spectrometric detection is summarized.<\/jats:p>","DOI":"10.1002\/bmc.5308","type":"journal-article","created":{"date-parts":[[2022,1,2]],"date-time":"2022-01-02T23:09:25Z","timestamp":1641164965000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["A review of chromatographic methods for bioactive tryptophan metabolites, kynurenine, kynurenic acid, quinolinic acid, and others, in biological fluids"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6086-4156","authenticated-orcid":false,"given":"Takeshi","family":"Fukushima","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences Toho University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maho","family":"Umino","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences Toho University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences Toho University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayu","family":"Onozato","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences Toho University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,31]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2021.108490"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)83608-0"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/prp2.741"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1178646919834551"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2017.04.010"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2021.462294"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-020-00421-4"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2019.03.005"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-018-3323-y"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(01)02242-X"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.786"},{"issue":"1","key":"e_1_2_13_13_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0022-3565(25)38782-3","article-title":"Kynurenic acid and quinolinic acid act at N\u2010methyl\u2010D\u2010aspartate receptors in the rat hippocampus","volume":"236","author":"Ganong A. 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