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The clinical reaction reports of <jats:styled-content style=\"fixed-case\"><jats:italic>S. tonkinensis<\/jats:italic><\/jats:styled-content> indicated its neurotoxicity; however, there still exists dispute about its toxic substances. At present, no report is available on the blood and brain prototype research of <jats:styled-content style=\"fixed-case\"><jats:italic>S. tonkinensis<\/jats:italic><\/jats:styled-content>. Most studies focused on alkaloids and less on other compounds. Moreover, the constituents absorbed into the blood and brain have been rarely investigated so far. This study established a rapid and efficient qualitative analysis method using UPLC\u2010Q\u2010TOF\u2010MS<jats:sup>E<\/jats:sup> to characterize the ingredients of <jats:styled-content style=\"fixed-case\"><jats:italic>S. tonkinensis<\/jats:italic><\/jats:styled-content> and those entering into the rat's body after oral administration. A total of 91 compounds were identified in <jats:styled-content style=\"fixed-case\"><jats:italic>S. tonkinensis<\/jats:italic><\/jats:styled-content>, of which 28 were confirmed by the standards. In addition, 30 and 19 prototypes were also first identified in the rat's blood and brain, respectively. It was found that most flavonoids, except alkaloids, were detected in the rat's body and distributed in the cerebrospinal fluid, suggesting that flavonoids may be one of the important toxic or effective substances of <jats:styled-content style=\"fixed-case\"><jats:italic>S. tonkinensis<\/jats:italic><\/jats:styled-content>. This finding provides new clues and data for clarifying the toxicity or efficacy of this medicinal plant.<\/jats:p>","DOI":"10.1002\/bmc.5357","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:15:11Z","timestamp":1645488911000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Systematic analysis of chemical profiles of <i>Sophorae tonkinensis<\/i> Radix et Rhizoma <i>in vitro<\/i> and <i>in vivo<\/i> using UPLC\u2010Q\u2010TOF\u2010MS<sup>E<\/sup>"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4759-8452","authenticated-orcid":false,"given":"Wen\u2010lin","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Natural Medicinal Chemistry, School of Pharmacy Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng\u2010rui","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry Xi'an University of Technology  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si\u2010jia","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Natural Medicinal Chemistry, School of Pharmacy Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Interdisciplinary Science Research Institute Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Natural Medicinal Chemistry, School of Pharmacy Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Natural Medicinal Chemistry, School of Pharmacy Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi\u2010ke","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Natural Medicinal Chemistry, School of Pharmacy Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2682-0755","authenticated-orcid":false,"given":"Xian\u2010peng","family":"Zu","sequence":"additional","affiliation":[{"name":"Department of Natural Medicinal Chemistry, School of Pharmacy Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8107-1593","authenticated-orcid":false,"given":"Yun\u2010heng","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Natural Medicinal Chemistry, School of Pharmacy Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786410701369557"},{"issue":"6","key":"e_1_2_9_3_1","first-page":"9737","article-title":"Effect of radix Sophorae Tonkinensis on the activity of cytochrome P450 isoforms in rats","volume":"8","author":"Cai J. 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