{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T18:38:29Z","timestamp":1789843109084,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,25]],"date-time":"2022-03-25T00:00:00Z","timestamp":1648166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100019033","name":"Key Research and Development Program of Liaoning Province","doi-asserted-by":"publisher","award":["2020JH2\/10300116"],"award-info":[{"award-number":["2020JH2\/10300116"]}],"id":[{"id":"10.13039\/501100019033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Schizophrenia is a debilitating psychiatric disorder affecting approximately 1% of the population worldwide. Disturbances in the homocysteine metabolism are an important factor in the pathophysiology of schizophrenia. In this research, a novel validated liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) quantification procedure was developed to investigate three significant compounds of homocysteine metabolism: homocysteine, cysteine, and methionine in patients with schizophrenia and healthy controls. Sample preparation involved a reduction with dithiothreitol followed by protein precipitation, and the chromatographic runtime was 2\u00a0min. The LC\u2013MS\/MS method was validated according to CLSI C62\u2010A and the Chinese Guidance for Liquid Chromatography and Mass Spectrometry Clinical Application. The performance of the method was excellent with a coefficient of variation for precision in the range of 0.5\u20136.9%, an accuracy of 90.4\u2013101.6%. In addition, the practical applicability of the method was demonstrated by applying it in the routine sample analysis for a schizophrenic patient. Increased homocysteine levels and decreased cysteine levels were observed in the patient with schizophrenia. These results indicate that the activity of the transsulfuration pathway may play a key role in the pathogenesis of schizophrenia.<\/jats:p>","DOI":"10.1002\/bmc.5366","type":"journal-article","created":{"date-parts":[[2022,3,10]],"date-time":"2022-03-10T23:09:40Z","timestamp":1646953780000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Simultaneous determination of serum homocysteine, cysteine, and methionine in patients with schizophrenia by liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1002","volume":"36","author":[{"given":"Zhenguo","family":"Li","sequence":"first","affiliation":[{"name":"The Second Hospital of Dalian Medical University  Dalian P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingli","family":"Liu","sequence":"additional","affiliation":[{"name":"Dalian Runsheng Kangtai Medical Lab Co. Ltd  Dalian P. R. 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