{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T20:02:57Z","timestamp":1789588977695,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T00:00:00Z","timestamp":1657670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This research developed and validated a highly sensitive and selective UPLC\u2013MS\/MS approach using a triple quadrupole mass spectrometer for quantifying favipiravir. Moreover, we introduced a study evaluating bioequivalence using two drugs, Favibrivix and Avigan, containing favipiravir. Lean Six Sigma verified the capacity and performance of the process. Protein precipitation extraction was utilized to extract favipiravir from the collected human matrices. We used an Acquity UPLCr BEH HILIC column and valproic acid as an internal standard. Furthermore, we conducted the procedure using an isocratic elution comprising acetonitrile and 0.005% ammonia in water (75:25, v\/v), a flow rate of 0.25\u2009ml\/min, a temperature controlled at 10\u00b0C and an injection volume of 1.0\u00a0\u03bcl. Our UPLC\u2013MS\/MS process has a broad range (50\u201310,000 ng\/ml) with a determination coefficient of 0.9980. We validated the method in line with the US Food and Drug Administration. The findings revealed that the test, Favibrivix 200\u2009mg\/tablet, and the reference, Avigan\u00ae 200\u2009mg\/tablet, were statistically bioequivalent in healthy Egyptian participants.<\/jats:p>","DOI":"10.1002\/bmc.5381","type":"journal-article","created":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T23:10:16Z","timestamp":1649373016000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Fully validated UPLC\u2013MS\/MS method for quantifying Favipiravir in human plasma boosted lean six sigma: An application for a bioequivalence study"],"prefix":"10.1002","volume":"36","author":[{"given":"Fathy Ibrahim","family":"Abd Allah","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics, Faculty of Pharmacy Al\u2010Azhar University  Cairo Egypt"},{"name":"International Center for Bioavailability, Pharmaceutical, and Clinical Research  Obour City Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Abdelhmaid","sequence":"additional","affiliation":[{"name":"International Center for Bioavailability, Pharmaceutical, and Clinical Research  Obour City Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmed","family":"Himida","sequence":"additional","affiliation":[{"name":"International Center for Bioavailability, Pharmaceutical, and Clinical Research  Obour City Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akram","family":"Mostafa Elkashlan","sequence":"additional","affiliation":[{"name":"International Center for Bioavailability, Pharmaceutical, and Clinical Research  Obour City Egypt"},{"name":"Biochemistry Department, Faculty of Pharmacy University of Sadat City  Sadat Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0012-6709","authenticated-orcid":false,"given":"Abdul\u2010Aziz M. 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