{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:50:54Z","timestamp":1760061054622,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T00:00:00Z","timestamp":1654473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Xiaopi granules have been shown to ameliorate gastric epithelial dysplasia in patients. However, the therapeutic mechanism is unclear. Herein, the proteomics method was applied to identify the differentially expressed proteins and related pathways. Sixty male Sprague\u2013Dawley rats were randomly divided into four groups: control (C group, <jats:italic>n<\/jats:italic>\u2009=\u200910), model (M group), Xiaopi granules (X group), and vitacoenzyme (V group). The rat gastric epithelial dysplasia model was established by intragastrically administering <jats:italic>N<\/jats:italic>\u2010methyl\u2010<jats:italic>N\u2032<\/jats:italic>\u2010nitro\u2010<jats:italic>N<\/jats:italic>\u2010nitrosoguanidine and ranitidine and by orally administering 0.05% ammonia solution. After 12\u2009weeks, the stomach tissue was analyzed by hematoxylin and eosin staining and proteomics analyses. Western\u2010blot analysis was applied to further validate the proteomics results. Compared to the M group, levels of 326 and 350 proteins were altered significantly in the X and V groups (1.5\u2010fold, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), which were significantly enriched in digestion, metabolism, coagulation, and cell apoptosis. CELA2A, GHRL, NDUFB9, and PGC were significantly upregulated (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001), whereas CLCA1, PLG, and DAC2 were downregulated (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001 or 0.0001) in the M group vs. the C group. The change in these proteins could be reversed after the treatment of Xiaopi granules or vitacoenzyme tablets. Xiaopi granules ameliorated gastric epithelial dysplasia by intervening in digestion, metabolism, blood coagulation, cell apoptosis, and other related pathways.<\/jats:p>","DOI":"10.1002\/bmc.5414","type":"journal-article","created":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T03:10:18Z","timestamp":1653275418000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proteomics analyses of Xiaopi granules in <i>N<\/i>\u2010methyl\u2010<i>N<\/i>\u2032\u2010nitro\u2010<i>N<\/i>\u2010nitrosoguanidine\u2013induced gastric epithelial dysplasia rat model using LC\u2013MS"],"prefix":"10.1002","volume":"36","author":[{"given":"Jisheng","family":"Peng","sequence":"first","affiliation":[{"name":"Department of traditional Chinese Medicine Peking University Shougang Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zehui","family":"Chen","sequence":"additional","affiliation":[{"name":"Graudate School, Beijing University of Chinese Medicine Beijing University of Chinese Medicine  Beijing China"},{"name":"Institute of Information on Traditional Chinese Medicine, Chinese Academy of Chinese Medical Sciences 100700  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huazheng","family":"Liang","sequence":"additional","affiliation":[{"name":"Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"Translational Research Institute of Brain and Brain\u2010Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3158-1149","authenticated-orcid":false,"given":"Jinxiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Spleen and Gastric Diseases Beijing University of Chinese Medicine Third Affiliated Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,6]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1645","article-title":"Intervention of Xiaopi granules in p16 methylation of rats with precancerous lesions of gastric cancer","volume":"14","author":"An J.","year":"2019","journal-title":"World Journal of Integrated Traditional and Western Medicine"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27908"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109427"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.04.013"},{"key":"e_1_2_9_7_1","first-page":"1628","article-title":"Effect of Xiaopi granules on miR\u201021\u2010PTEN\u2010PI3K\/AKT signaling pathway in gastric mucosatissue of precancerous lesions of gastric cancer model rats","volume":"61","author":"Chen Z. 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