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Azithromycin (AZI) is the most common outpatient prescribed antibiotic in the US and clinical studies have demonstrated the efficacy and safety of AZI in many bacterial infections. To support a clinical study, we developed a high\u2010throughput LC\u2013MS\/MS method to process up to 250 samples per day to quantify AZI in human plasma. Samples were prepared by solid\u2010phase extraction. Separation was achieved with an ACE C<jats:sub>18<\/jats:sub> column (2.1\u2009\u00d7\u2009100\u2009mm, 1.7\u00a0\u03bcm) equipped with a C<jats:sub>18<\/jats:sub> guard column. The mobile phase consisted of 0.1% formic acid and methanol\u2013acetonitrile (1:1, v\/v) at a flow rate of 0.25\u2009ml\/min. The ionization was optimized with positive electrospray source using multiple reaction monitoring transition, <jats:italic>m\/z<\/jats:italic> 749.50\u2009&gt;\u2009591.45 for AZI and <jats:italic>m\/z<\/jats:italic> 754.50\u2009&gt;\u2009596.45 for AZI\u2010d5. Extraction recoveries were approximately 90% for AZI. The assay was linear from 0.5 to 2,000\u2009ng\/ml and required only 100\u2009\u03bcl of plasma with a total analysis time of 4.5\u00a0min. The method was successfully applied to pharmacokinetic studies of a weight\u2010based dosing protocol for AZI.<\/jats:p>","DOI":"10.1002\/bmc.5443","type":"journal-article","created":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T06:46:06Z","timestamp":1657003566000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A simple, high\u2010throughput and validated LC\u2013MS\/MS method for the determination of azithromycin in human plasma and its application to a clinical pharmacokinetic study"],"prefix":"10.1002","volume":"36","author":[{"given":"Yuning","family":"Zhang","sequence":"first","affiliation":[{"name":"Clinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, College of Pharmacy University of Nebraska Medical Center  Omaha NE USA"},{"name":"Department of Pharmacy, Nanfang Hospital Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veenu","family":"Bala","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, College of Pharmacy University of Nebraska Medical Center  Omaha NE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6455-5388","authenticated-orcid":false,"given":"Yashpal S.","family":"Chhonker","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, College of Pharmacy University of Nebraska Medical Center  Omaha NE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wafaa N.","family":"Aldhafiri","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, College of Pharmacy University of Nebraska Medical Center  Omaha NE USA"},{"name":"Department of Pharmaceutical Science, College of Pharmacy University of Nebraska Medical Center  Omaha NE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy N.","family":"John","sequence":"additional","affiliation":[{"name":"National Department of Health  Port Moresby Papua New Guinea"},{"name":"University of Barcelona  Barcelona Spain"},{"name":"School of Medicine and Health Sciences University of Papua New Guinea  Port Moresby Papua New Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine M.","family":"Bjerum","sequence":"additional","affiliation":[{"name":"Center for Global Health and Diseases Case Western Reserve University  Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher L.","family":"King","sequence":"additional","affiliation":[{"name":"Center for Global Health and Diseases Case Western Reserve University  Cleveland OH USA"},{"name":"Veterans Affairs Medical Center  Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriol","family":"Mitja","sequence":"additional","affiliation":[{"name":"University of Barcelona  Barcelona Spain"},{"name":"School of Medicine and Health Sciences University of Papua New Guinea  Port Moresby Papua New Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Marks","sequence":"additional","affiliation":[{"name":"London School of Hygiene and Tropical Medicine  London UK"},{"name":"Hospital for Tropical Diseases  London UK"},{"name":"Division of Infection and Immunity University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daryl J.","family":"Murry","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, College of Pharmacy University of Nebraska Medical Center  Omaha NE USA"},{"name":"Fred and Pamela Buffett Cancer Center University of Nebraska Medical Center  Omaha NE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3329\/dujps.v11i1.12488"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh519"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.03.042"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","unstructured":"Barrett B. 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