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This study aimed to develop and validate an LC\u2013ESI\u2013QTOF\u2013MS method for the quantification of cefepime in the plasma and peritoneal microdialysate of healthy Wistar rats. Chromatographic separation was performed using a CLC\u2010ODS C<jats:sub>18<\/jats:sub> column (250\u2009\u00d7\u00a04.6\u00a0mm), a C<jats:sub>18<\/jats:sub> pre\u2010column (4\u00a0mm, 5\u00a0\u03bcm) and isocratic elution. Gallic acid was used as the internal standard. The mobile phase consisted of (A) ultrapure water (pH adjusted to 3.5) and (B) acetonitrile (80:20, v\/v) at 0.8\u00a0ml\/min. Quantification was performed using a mass spectrometer with electrospray ionization in positive mode to monitor ions with <jats:italic>m\/z<\/jats:italic> 481.1322 (CEF) and <jats:italic>m\/z<\/jats:italic> 171.0288 (IS). The method was validated for selectivity, precision, accuracy, linearity, stability, lower limit of quantification, carryover, recovery and matrix effect. Calibration was done in the ranges 1\u201340\u2009and 1\u2013100\u2009\u03bcg\/ml for the peritoneal microdialysate and plasma, respectively. Plasma extraction recovery ranged from 93.9 to 99.9%. The technique was validated and successfully applied in a pilot pharmacokinetic study for estimating the free concentration of CEF in the peritoneal microdialysate of rats for the first time.<\/jats:p>","DOI":"10.1002\/bmc.5470","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T09:24:23Z","timestamp":1659086663000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development and validation of an LC\u2013ESI\u2013QTOF\u2013MS method to measure cefepime in the plasma and peritoneal fluid of rats using microdialysis: Application in a pilot pharmacokinetic study"],"prefix":"10.1002","volume":"36","author":[{"given":"Michele Vaz","family":"dos Anjos","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Farmacocin\u00e9tica, Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade Universidade de Caxias do Sul  Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduarda","family":"Possa","sequence":"additional","affiliation":[{"name":"Curso de Farm\u00e1cia Universidade de Caxias do Sul  Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisele","family":"da Silva Fonseca","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Biotecnologia Universidade de Caxias do Sul  Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa","family":"Bergoza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Farmacocin\u00e9tica, Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade Universidade de Caxias do Sul  Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Roberto","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Biotecnologia Universidade de Caxias do Sul  Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sidnei","family":"Moura e Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Biotecnologia Universidade de Caxias do Sul  Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7102-9204","authenticated-orcid":false,"given":"Leandro","family":"Tasso","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Farmacocin\u00e9tica, Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade Universidade de Caxias do Sul  Rio Grande do Sul Brazil"},{"name":"Curso de Farm\u00e1cia Universidade de Caxias do Sul  Rio Grande do Sul Brazil"},{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Biotecnologia Universidade de Caxias do Sul  Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00595-18"},{"key":"e_1_2_8_3_1","unstructured":"Barshak M. 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