{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T19:42:11Z","timestamp":1770061331936,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960757"],"award-info":[{"award-number":["81960757"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia","doi-asserted-by":"publisher","award":["2021MS08160"],"award-info":[{"award-number":["2021MS08160"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study established the fingerprint of <jats:italic>Syringa pinnatifolia<\/jats:italic> Hemsl. (SP), analyzed the SP ingredients absorbed into the rats blood, and evaluated its anti\u2010myocardial ischemic effect to provide a scientific basis for the follow\u2010up development and research of SP and lay a foundation for its clinical application using ultra\u2010performance liquid chromatography\u2010Q Exactive\u2010mass spectrometry and GC\u2013MS. Myocardial infarction was induced in rat by ligating the left anterior descending branch of the rat coronary artery, and SP alcohol extract was administered to evaluate its anti\u2010myocardial ischemic effect. We analyzed the SP ingredients absorbed into the rats blood, screened the active compounds, established a database of SP anti\u2010myocardial ischemic targets, and explored the possible mechanism of SP in treating myocardial infarction using bioinformatics. The rats were examined using echocardiography, serum biomarkers were determined, and pathological changes were observed by histopathological examination. TUNEL staining was performed to detect the apoptotic level of cells, and Western blot and quantitative real\u2010time polymerase chain reaction were performed to detect the expression levels of Bcl\u20102, Bax, and Caspase\u20103 in heart tissues. In the fingerprint of SP, 24 common peaks were established, and the similarity evaluation results of 10 batches of SP were all &gt;0.9. Ultra\u2010performance liquid chromatography\u2010Q Exactive\u2010mass spectrometry and GC\u2013MS detected 17 active ingredients in the drug\u2010containing serum, including terpenoids, flavonoids, phenols, phenylpropanoids, and phenolic acids, the most abundant of which was resveratrol. Enrichment analysis of SP targets against myocardial ischemia revealed that key candidate targets of SP were significantly enriched in multiple pathways associated with apoptosis. Resveratrol was administered to the successfully modeled rats, and the results showed that the resveratrol group significantly decreased left ventricular end\u2010diastolic diameter and left ventricular end\u2010systolic diameter and significantly increased ejection fraction and fractional shortening in all groups compared with the model group. Resveratrol significantly decreased the levels of creatine kinase isoenzyme and lactate dehydrogenase in serum compared to the model group (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Hematoxylin\u2013eosin staining of rat myocardial tissue showed that all lesions were reduced under microscopic observation in the resveratrol group compared with the model group. Real\u2010time polymerase chain reaction and Western blot results showed that the resveratrol group downregulated the expression of the proapoptotic factor Bax, upregulated the expression of the antiapoptotic factor Bcl\u20102, and decreased the expression of Caspase\u20103. The established fingerprints are accurate, reliable, and reproducible and can be used as an effective method for quality control of the herbs. The anti\u2010myocardial ischemia effect of SP is that resveratrol improves cardiac function and inhibits cardiomyocyte apoptosis to protect cardiomyocytes. The present study provides ample evidence for the clinical use of SP, suggesting that this drug has great potential in the treatment of ischemic heart disease.<\/jats:p>","DOI":"10.1002\/bmc.5475","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T10:04:12Z","timestamp":1660125852000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Establishment of fingerprint and mechanism of anti\u2010myocardial ischemic effect of <i>Syringa pinnatifolia<\/i>"],"prefix":"10.1002","volume":"36","author":[{"given":"Ye","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Pharmacy Inner Mongolia Medical University  Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingkun","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Basic Medicine Inner Mongolia Medical University  Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuheng","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Pharmacy Inner Mongolia Medical University  Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijun","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Pharmacy Inner Mongolia Medical University  Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suwei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Inner Mongolia Medical University  Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Yue","sequence":"additional","affiliation":[{"name":"College of Pharmacy Inner Mongolia Medical University  Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiuwang","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Pharmacy Inner Mongolia Medical University  Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2927-6855","authenticated-orcid":false,"given":"Peifeng","family":"Xue","sequence":"additional","affiliation":[{"name":"College of Pharmacy Inner Mongolia Medical University  Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-022-04598-x"},{"key":"e_1_2_8_3_1","first-page":"4196","article-title":"An updated phytochemical and pharmacological progress on Syringa pinnatifolia","volume":"45","author":"Chang\u2010Xin L.","year":"2020","journal-title":"Zhongguo Zhong Yao Za Zhi"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.12942"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115106"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2018.06.006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/2647807"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-022-03537-5"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/2211-5463.13097"},{"issue":"16","key":"e_1_2_8_10_1","first-page":"3797","article-title":"Microscopic histochemical comparison during mountain\u2010agarwood formation","volume":"45","author":"Gao J.\u2010Q.","year":"2020","journal-title":"Zhongguo Zhong Yao Za Zhi"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmab012"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-06811-6"},{"key":"e_1_2_8_13_1","first-page":"5240","article-title":"Pharmacological evaluation of Mongolian medicine Syringa pinnatifolia fraction I against acute myocardial ischemia in mice","volume":"44","author":"Li J. 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