{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T14:27:16Z","timestamp":1763389636860,"version":"build-2065373602"},"reference-count":21,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T00:00:00Z","timestamp":1661299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The study objective was to analyse the phytochemical constituents in aerial extracts of these plants using an HPTLC method and optimization by quality by design. Qualitative analysis of ephedrine in hydro\u2010alcoholic extract was done via HPTLC, using a mobile phase of toluene\u2013ethyl acetate\u2013chloroform\u2013formic acid in the ratio of 1:0.5:0.5:01 and the peaks were monitored at 366\u2009nm. In the hydro\u2010alcoholic aerial part extract, ephedrine was identified using the HPTLC method and the retardation factor (Rf) value was found to be 0.69 \u00b1 0.01 and 0.69 \u00b1 0.01, as compared with the standard sample. The extraction of plant materials was done using different concentration of water and alcohol solvents and quality by design was applied to optimize the extraction process and to find out the best extraction in an 80:20 ratio of hydro\u2010alcoholic extract. In the hydro\u2010alcoholic extract, the ephedrine was characterized using the HPTLC method and compared with the standard solution, and this method was used in herbal as well as academic research for the identification of ephedrine in poly herbal formulations as well as the ephedrine present in different plant extracts. Response surface methodology software was utilized to predict the path or choose the best extraction method. <jats:styled-content style=\"fixed-case\"><jats:italic>Sida rhombifolia<\/jats:italic><\/jats:styled-content> <jats:italic>and <jats:styled-content style=\"fixed-case\">Sida cordifolia<\/jats:styled-content><\/jats:italic> can be used as substitutes for <jats:styled-content style=\"fixed-case\"><jats:italic>Ephedra gerardiana<\/jats:italic><\/jats:styled-content> based on the HPTLC profile.<\/jats:p>","DOI":"10.1002\/bmc.5479","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T18:43:18Z","timestamp":1660329798000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Quality by design\u2010based optimization of Soxhlet extraction and identification of ephedrine by a HPTLC method for <scp><i>Sida rhombifolia<\/i><\/scp> and <scp><i>Sida Cordifolia<\/i><\/scp>"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7349-3019","authenticated-orcid":false,"given":"Mohd","family":"Imran","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research Jamia Hamdard University, Hamdard Nagar  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shital Pradeep","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research Jamia Hamdard University, Hamdard Nagar  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed A. S.","family":"Abourehab","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, College of Pharmacy Umm Al\u2010Qura University  Makkah Saudi Arabia"},{"name":"Department of Pharmaceutics and Industrial Pharmacy, College of Pharmacy Minia University  Minia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vidhu","family":"Aeri","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research Jamia Hamdard University, Hamdard Nagar  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0890-769X","authenticated-orcid":false,"given":"Prashant","family":"Kesharwani","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmaceutical Education and Research Jamia Hamdard, Hamdard Nagar  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/pm.pm_609_18"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.30492\/IJCCE.2016.23556"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/pm.pm_609_18"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"6376\u20136393","DOI":"10.20964\/2019.07.02","article-title":"Influence of phenolic compounds on antioxidant and anticorrosion activities of Ammi visnaga extracts obtained ultrasonically in three solvent systems","volume":"14","author":"Aourabi S.","year":"2019","journal-title":"International Journal of Electrochemical Science"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1951.tb00067.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2403"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.10.027"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(75)83057-3"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/js9801844"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2795"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr7100749"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/pm.pm_295_20"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22111883"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221-1691(14)60202-1"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1296.157722"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2014.11.002"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-812364-5.00001-8"},{"volume-title":"Malvaceae of southern peninsular India: A taxonomic monograph","year":"1996","author":"Sivarajan V.","key":"e_1_2_8_19_1"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijfs.12413"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2010.01.165"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1296.141754"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5479","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.5479","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5479","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:50:43Z","timestamp":1760043043000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,24]]},"references-count":21,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/bmc.5479"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5479","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2022,8,24]]},"assertion":[{"value":"2022-06-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5479"}}