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Cuscutae semen (CS), a widely used Chinese medicine, is a tonic to nourish the liver and kidney. Our research aimed to assess the hepatoprotective effect of CS on CCl<jats:sub>4<\/jats:sub>\u2010induced liver injury rats using plasma metabolomics. Liver injury in rats was induced by 40% CCl<jats:sub>4<\/jats:sub> in olive oil twice a week for 21\u2009days. The CS group received 2\u2009g\/kg of CS every day for 21\u2009days. The liver tissues were used for histological studies. The serum was used for the analysis of biochemical parameters. Plasma metabolomic analysis was performed using ultra\u2010performance liquid chromatography coupled with quadrupole time\u2010of\u2010flight mass spectrometry. CS could relieve hepatocyte necrosis and decrease the levels of serum biochemical parameters in comparison the with CCl<jats:sub>4<\/jats:sub> group. Principal component analysis and orthogonal partial least squares\u2010discriminant analysis on plasma metabolomes showed an obvious separation among the control, model, and CS groups. Heatmap showed that CS\u2010administered mice had similar metabolite profiles as the control group. Seven influential pathways in plasma of the hepatoprotective effect impacted by CS were identified. This study confirmed the hepatoprotective effect of CS, and the related metabolic pathways were discussed.<\/jats:p>","DOI":"10.1002\/bmc.5489","type":"journal-article","created":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T00:09:05Z","timestamp":1661386145000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ultra\u2010performance liquid chromatography quadrupole time\u2010of\u2010flight mass spectrometry\u2013based plasma metabolomics study of hepatoprotective effect of cuscutae semen on CCl<sub>4<\/sub>\u2010induced liver injury model of rats"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3109-8368","authenticated-orcid":false,"given":"Shu\u2010ya","family":"Xu","sequence":"first","affiliation":[{"name":"School of Animal Medicine Henan University of Animal Husbandry and Economy  Zhengzhou China"},{"name":"School of Chinese Materia Medica Beijing University of Chinese 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