{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T06:46:26Z","timestamp":1785221186941,"version":"3.55.0"},"reference-count":11,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A sensitive, selective and rapid bioanalytical method using liquid chromatography\u2013tandem mass spectrometry has been developed for the quantification of trifluoperazine in human plasma. Trifluoperazine\u2010D<jats:sub>8<\/jats:sub> was used as the internal standard and the extraction from human plasma was performed by liquid\u2013liquid extraction technique using tertiary butyl methyl ether as the solvent. Chromatographic separation was carried out on Zodiac C<jats:sub>18<\/jats:sub> column (50\u2009\u00d7\u20094.6\u00a0mm, 3\u00a0\u03bcm) employing a mixture of acetonitrile, methanol and 5\u00a0m<jats:sc>m<\/jats:sc> ammonium bicarbonate buffer in water (85:10:5, v\/v\/v) at a flow rate of 0.55\u2009ml\/min. The linearity was established within the concentration range of 5\u20131,250\u2009pg\/ml with <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u2009&gt;\u20090.99. The results of all of the validation parameters as per the US Food and Drug Administration guidelines were within the acceptance limits. The pharmacokinetics of trifluoperazine after oral administration of a syrup of 1\u00a0mg dose under fasting conditions was determined by successful application of the present method.<\/jats:p>","DOI":"10.1002\/bmc.5499","type":"journal-article","created":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T23:03:40Z","timestamp":1662419020000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Bioanalysis of trifluoperazine in human plasma by LC\u2013MS\/MS: Application to disposition kinetics"],"prefix":"10.1002","volume":"36","author":[{"given":"Viritha","family":"Bezawada","sequence":"first","affiliation":[{"name":"Aurora Degree and PG College  Hyderabad Telangana India"},{"name":"Department of Engineering Chemistry, College of Engineering Andhra University  Visakhapatnam Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Padma","family":"Mogili","sequence":"additional","affiliation":[{"name":"Department of Engineering Chemistry, College of Engineering Andhra University  Visakhapatnam Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9315-9085","authenticated-orcid":false,"given":"Sireesha","family":"Dodda","sequence":"additional","affiliation":[{"name":"Anurag University  Hyderabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramakrishna","family":"Gajula","sequence":"additional","affiliation":[{"name":"VLS Clinical Research Organisation  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhasini","family":"Ponnada","sequence":"additional","affiliation":[{"name":"Aurobindo Pharma Ltd.  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