{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T03:21:27Z","timestamp":1789096887137,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:00:00Z","timestamp":1663632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["18\u2010CE09\u20100042"],"award-info":[{"award-number":["18\u2010CE09\u20100042"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017007","name":"Agence R\u00e9gionale de Sant\u00e9 \u00cele-de-France","doi-asserted-by":"publisher","award":["SYNALGIA"],"award-info":[{"award-number":["SYNALGIA"]}],"id":[{"id":"10.13039\/501100017007","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inborn errors of monoamine neurotransmitter metabolism are rare genetic diseases classified as catecholamine and serotonin metabolism disorders or neurotransmitter transportopathies. To diagnose these orphan diseases, monoamine metabolites have been identified and validated as cerebrospinal fluid (CSF) biomarkers: 5\u2010hydroxy\u2010tryptophane, 5\u2010hydroxy\u2010indol\u2010acetic acid, 3\u2010<jats:italic>ortho<\/jats:italic>\u2010methyl\u2010DOPA, homovanillic acid, and 3\u2010methoxy\u20104\u2010hydroxyphenylglycol. The present work presents a UHPLC\u2013MS\/MS method developed for the quantification of these metabolites in CSF and compares it with a previously described UHPLC with fluorescence detection (UHPLC\u2010FD) method. MS\/MS detection was performed in positive electrospray ionization and multiple reaction monitoring mode. The UHPLC\u2013MS\/MS and UHPLC\u2013FD methods were validated in terms of accuracy, linearity, precision and matrix effect. The lower limits of quantification (LLOQ) ranged between 0.5\u00a0and 10\u00a0n<jats:sc>m<\/jats:sc> and between 1 and 5\u00a0n<jats:sc>m<\/jats:sc> for the UHPLC\u2013MS\/MS method and the UHPLC\u2013FD one, respectively. We verified the applicability of both methods by analyzing 30 CSF samples. The measured concentrations were comparable with the reference values described in the literature. The two methods allowed pathological samples to be distinguished from healthy ones for clinical diagnosis. UHPLC\u2013MS\/MS and UHPLC\u2013FD methods exhibited very close LLOQs. As the UHPLC\u2013MS\/MS method is more selective, it allows faster analysis with a run time of 6\u00a0min per run vs. 10\u00a0min for the UHPLC\u2013FD method.<\/jats:p>","DOI":"10.1002\/bmc.5502","type":"journal-article","created":{"date-parts":[[2022,9,9]],"date-time":"2022-09-09T03:16:08Z","timestamp":1662693368000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Quantification of monoamine biomarkers in cerebrospinal fluid: Comparison of a UHPLC\u2013MS\/MS method with a UHPLC coupled to fluorescence detection method"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0240-0636","authenticated-orcid":false,"given":"Ayoub","family":"Boulghobra","sequence":"first","affiliation":[{"name":"CNRS, Institut de Chimie Physique Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taous","family":"Abar","sequence":"additional","affiliation":[{"name":"CNRS, Institut de Chimie Physique Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fathi","family":"Moussa","sequence":"additional","affiliation":[{"name":"CNRS, Institut de Chimie Physique Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Baudin","sequence":"additional","affiliation":[{"name":"Services de Neurop\u00e9diatrie et de Biochimie Groupe Hospitalier Trousseau Laroche\u2010Guyon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Services de Neurop\u00e9diatrie et de Biochimie Groupe Hospitalier Trousseau Laroche\u2010Guyon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Pallandre","sequence":"additional","affiliation":[{"name":"CNRS, Institut de Chimie Physique Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Bonose","sequence":"additional","affiliation":[{"name":"CNRS, Institut de Chimie Physique Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2016.12.005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.04.007"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1781(81)90057-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2012.12.030"},{"key":"e_1_2_7_6_1","unstructured":"Bioanalytical method validation. (2018 septembre 17).[Text]. European Medicines Agency.https:\/\/www.ema.europa.eu\/en\/bioanalytical-method-validation"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450\u2010199804000\u201000006"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123264"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1673630"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.102.6.e69"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2019.05.011"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461775"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-014-9723-6"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/med\/9780199972135.001.0001"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0146-0005(99)80051-2"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2007.099986"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199307000-00003"},{"key":"e_1_2_7_18_1","volume-title":"StatPearls","author":"Jeanmonod R.","year":"2022"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8080867"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(11)70141-7"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-017-0079-6"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.7b01008"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000003897"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-014-9697-4"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40272-014-0079-z"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgmr.2016.09.006"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2004.08.007"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/0883073807302619"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1207281"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.31887\/DCNS.2018.20.4\/jmsaudubray"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-007-0661-4"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(85)90242-5"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-013-9618-y"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgmr.2021.100762"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5502","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.5502","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5502","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:50:59Z","timestamp":1760043059000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5502"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,20]]},"references-count":33,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/bmc.5502"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5502","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,20]]},"assertion":[{"value":"2022-06-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5502"}}