{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T19:42:42Z","timestamp":1778960562789,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T00:00:00Z","timestamp":1664496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The current research work describes the development of a rapid HPLC method for the concurrent detection of pregabalin and piperine in dual drug\u2010loaded nanoformulations. The primary goal was to recognize the chromatographic conditions wherein propitious segregation of the integrants with quality peaks can be attained. An attempt to expound the target analytical profile was made to accomplish this goal, and critical method attributes (CMAs), viz. percentage acetonitrile content, injection volume and pH, which affect critical quality attributes (CQAs), were identified using systemic risk analysis. Box\u2013Behnken design was employed to develop a relationship between CMAs and CQAs, which engenders an analytical design space. Efficient chromatographic separation for pregabalin and piperine was attained using an analytical C<jats:sub>18<\/jats:sub> column and mobile phase comprising acetonitrile\u2013water (pH\u00a06.9; 70:30%, v\/v) in an isocratic elution mode with a 1\u00a0ml\/min flow rate. The elution was descried at an isosbestic wavelength of 221\u2009nm using a photodiode array detector. The International Conference on Harmonization guidelines were adopted for the developed HPLC method. The validated HPLC method can be further utilized for the simultaneous quantification and detection of pregabalin and piperine in other lipid\u2010based nanopharmaceuticals such as polymeric nanoparticles, nanocrystals, solid\u2010lipid nanoparticles, metallic nanoparticles, etc., in <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> studies.<\/jats:p>","DOI":"10.1002\/bmc.5510","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T23:10:10Z","timestamp":1663110610000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Quality by design\u2010based development and validation of an HPLC method for simultaneous estimation of pregabalin and piperine in dual drug\u2010loaded liposomes"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2987-2967","authenticated-orcid":false,"given":"Isha","family":"Gupta","sequence":"first","affiliation":[{"name":"Phytomedicine Laboratory, Department of Pharmacognosy &amp; Phytochemistry School of Pharmaceutical Education &amp; Research, Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7857-1124","authenticated-orcid":false,"given":"Syeda Nashvia","family":"Adin","sequence":"additional","affiliation":[{"name":"Phytomedicine Laboratory, Department of Pharmacognosy &amp; Phytochemistry School of Pharmaceutical Education &amp; Research, Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6797-3104","authenticated-orcid":false,"given":"Mohd.","family":"Aqil","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics School of Pharmaceutical Education &amp; Research, Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7204-780X","authenticated-orcid":false,"given":"Mohd.","family":"Mujeeb","sequence":"additional","affiliation":[{"name":"Phytomedicine Laboratory, Department of Pharmacognosy &amp; Phytochemistry School of Pharmaceutical Education &amp; Research, Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasmin","family":"Sultana","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics School of Pharmaceutical Education &amp; Research, Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2019.1667820"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2662"},{"key":"e_1_2_7_4_1","first-page":"179","article-title":"QbD approach to analytical method development and its validation","volume":"3","author":"Bhatt D.","year":"2011","journal-title":"International Journal of Pharmacy and Pharmaceutical Sciences"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2016.11.013"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-014-0832-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40263-014-0192-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/03639045.2019.1660366"},{"key":"e_1_2_7_9_1","unstructured":"Guideline IHT. 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