{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:23:16Z","timestamp":1791292996325,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T00:00:00Z","timestamp":1664150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073558"],"award-info":[{"award-number":["82073558"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tryptophan (TRP) and its metabolites exhibit significant biological effects and are strongly associated with age\u2010related disease and mortality. However, reports on quantitatively analyzing these metabolites in older individuals are not available. We used ultra\u2010high\u2010performance liquid chromatography\u2013tandem mass spectrometry to optimize and validate a method for isotope dilution analysis of TRP metabolites in older individuals. The targeted analytes are TRP, serotonin or 5\u2010hydroxytryptamine, kynurenine, kynurenic acid, xanthurenic acid, indole\u20103\u2010acetic acid, indole\u20103\u2010propionic acid, and tryptamine. The serum sample was purified using solid\u2010phase extraction and was separated on a Waters HSS T3 column (100 mm\u2009\u00d7\u20092.1\u2009mm, 1.8\u2009\u03bcm). The analytes were detected in the multiple reaction monitoring mode under positive ionization. TRP was confirmed and measured after being diluted 100 times. This method exhibited satisfactory linearity (<jats:italic>r<\/jats:italic>\u2009&gt;\u20090.99). The intrabatch and interbatch accuracies (85.7\u2013114%) and precisions (&lt;15%) were acceptable. The standard\u2010normalized matrix effects ranged from 51.6 to 145%. This method was successfully applied to a cohort of 1021 older Chinese individuals, and this study may enable further understanding of the metabolic phenotypes associated with TRP in other populations.<\/jats:p>","DOI":"10.1002\/bmc.5512","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T02:43:42Z","timestamp":1663123422000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Simultaneous determination of serum tryptophan metabolites in an older Chinese population"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9028-5588","authenticated-orcid":false,"given":"Sheng","family":"Wang","sequence":"first","affiliation":[{"name":"Center for Scientific Research of Anhui Medical University Anhui Medical University  Hefei China"},{"name":"Anhui Province Key Laboratory of Major Autoimmune Diseases  Hefei 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