{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T15:18:16Z","timestamp":1776784696251,"version":"3.51.2"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:00:00Z","timestamp":1667520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we aimed to demonstrate the therapeutic effect of Ligustri Lucidi Fructus on chemotherapy\u2010induced myelosuppression and elucidate its mechanism. A pharmacological study was conducted to investigate the mechanism of the inhibiting effects of Ligustri Lucidi Fructus on cyclophosphamide\u2010induced bone marrow suppression in mice. HPLC was used to measure the chemical components. We demonstrated that medium and high doses of Ligustri Lucidi Fructus increased the amount of white blood cells and bone marrow nucleated cells (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in the cyclophosphamian\u2010induced mouse model, and at the same time reduced granulocyte\u2013macrophage\u2013colony stimulating factor and thrombopoietin in the serum of myelosuppression mice (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Medium and high doses of Ligustri Lucidi Fructus can also adjust the thymus index and spleen index(<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Ligustri Lucidi Fructus regulates the balance of bcl\u20102\/bax, inhibits the expression of Caspase\u20103 and meanwhile stimulates the expression of mitogen\u2010activated protein (MEK) and phospho extracellular regulated protein kinases (p\u2010ERK) on the MAPK pathway. Five chemical constituents of Ligustri Lucidi Fructus, which may be related to myelosuppression, were analyzed. The content of specnuezhenide was 0.281%, that of ligustroflavone was 0.004%, that of salidroside was 0.094%, that of hydroxytyrosol was 0.060% and that of tyrosol was 0.069%. The effect of Ligustri Lucidi Fructus on myelosuppression after chemotherapy may be related to its multicomponent and multitarget nature. Ligustri Lucidi Fructus may be a promising potential drug for treatment after chemotherapy.<\/jats:p>","DOI":"10.1002\/bmc.5524","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T20:18:11Z","timestamp":1665778691000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of Ligustri Lucidi Fructus on myelosuppression in mice induced by cytoxan"],"prefix":"10.1002","volume":"37","author":[{"given":"Jiahong","family":"Han","sequence":"first","affiliation":[{"name":"College of Chinese Medicinal Material Jilin Agricultural University  Changchun Jilin province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Fei","sequence":"additional","affiliation":[{"name":"College of Chinese Medicinal Material Jilin Agricultural University  Changchun Jilin province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nian","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Chinese Medicinal Material Jilin Agricultural University  Changchun Jilin province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junjia","family":"Xing","sequence":"additional","affiliation":[{"name":"College of Chinese Medicinal Material Jilin Agricultural University  Changchun Jilin province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6355-7071","authenticated-orcid":false,"given":"Enbo","family":"Cai","sequence":"additional","affiliation":[{"name":"College of Chinese Medicinal Material Jilin Agricultural University  Changchun Jilin province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Limin","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Chinese Medicinal Material Jilin Agricultural University  Changchun Jilin province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,11,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0048-9697(03)00140-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(98)00006-5"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2255-17.2017"},{"issue":"8","key":"e_1_2_9_5_1","first-page":"32","article-title":"Advances in MAPK signaling pathways","volume":"01","author":"Chen J. 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