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Considering that immunocompromised lung cancer patients are prone to opportunistic fungal infections, and most triazole antifungal drugs are moderate or strong inhibitors of CYP3A4, this study was conducted to develop and validate an accurate and rapid ultra\u2010performance liquid chromatography tandem mass spectrometry method for quantifying almonertinib in plasma and for investigating the pharmacokinetic changes of almonertinib caused by voriconazole and fluconazole in rats. After liquid\u2013liquid extraction with <jats:italic>tert<\/jats:italic>\u2010butyl methyl ether, an XSelect HSS T3 column (2.1\u2009\u00d7\u2009100\u2009mm, 2.5\u00a0\u03bcm, Waters) was used for the chromatographic separation of almonertinib and sorafenib\u2010D<jats:sub>3<\/jats:sub> (internal standard). The analytes were detected using an AB Sciex Triple Quad 5,500 mass spectrometer in the positive ionization mode. The method exhibited great linearity (0.5\u2013200\u2009ng\/ml, <jats:italic>r<\/jats:italic>\u2009&gt;\u20090.997) and stability under the established experimental conditions. All validation experiments were in accordance with the guidelines, and the results were all within the acceptable limits. This method was successfully applied to the researches of pharmacokinetics and drug interactions for almonertinib in rats. Voriconazole and fluconazole significantly altered the pharmacokinetic profiles of almonertinib and increased the systemic exposure of almonertinib in rats to different degrees, but further human trials should be conducted to validate the results.<\/jats:p>","DOI":"10.1002\/bmc.5525","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T21:35:01Z","timestamp":1665783301000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of voriconazole and fluconazole on the pharmacokinetics of almonertinib in rats by UPLC\u2013MS\/MS"],"prefix":"10.1002","volume":"37","author":[{"given":"Yuhao","family":"Fu","sequence":"first","affiliation":[{"name":"Graduate School of Hebei Medical University  Shijiazhuang China"},{"name":"Department of Pharmacy Hebei General Hospital  Shijiazhuang 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