{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:36:27Z","timestamp":1787726187308,"version":"build-2784847793"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7192218"],"award-info":[{"award-number":["7192218"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tacrolimus (TAC) and sirolimus (SIR) antirejection medications are widely used in organ transplantation. We aimed to develop an ultra\u2010high performance liquid chromatography tandem mass spectrometry (UHPLC\u2013MS\/MS) assay for quantifying TAC and SIR simultaneously and evaluating agreement with chemiluminescence microparticle immunoassay (CMIA) and electrochemiluminescence immunoassay (ECLIA). Whole blood samples collected from 209 TAC and 208 SIR patients were assessed by UHPLC\u2013MS\/MS, CMIA and ECLIA. The agreement of the three techniques was assessed using the Bland\u2013Altman plot. The UHPLC\u2013MS\/MS assay had a calibration range of 1\u2013100\u2009ng\/ml for TAC and SIR. The accuracy and precision were \u22122.73\u20134.32% and &lt;4.71% for TAC, respectively, and 0.07\u20134.84% and &lt;6.5% for SIR, respectively. The three methods had good correlation. In comparison with UHPLC\u2013MS\/MS, two immunoassays showed a slight deviation in proportion. An UHPLC\u2013MS\/MS method for simultaneously detecting TAC and SIR in human whole blood was developed, validated and comparatively analyzed with CMIA and ECLIA. For determining TAC and SIR, immunoassays displayed acceptable analytical performances in terms of precision and correlation compared with UHPLC\u2013MS\/MS. However, further investigation is warranted to examine the novel method.<\/jats:p>","DOI":"10.1002\/bmc.5538","type":"journal-article","created":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T10:29:10Z","timestamp":1666434550000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of UHPLC\u2013MS\/MS method for simultaneous determination of tacrolimus and sirolimus in human whole blood and comparisons with two immunoassays"],"prefix":"10.1002","volume":"37","author":[{"given":"Boyu","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pharmacy Peking University People's Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Peking University People's Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Peking University People's Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiran","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Peking University People's Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Peking University People's Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3264-1374","authenticated-orcid":false,"given":"Lin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Peking University People's Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Peking University People's Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5071"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2011.010"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2018.11.026"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2006.05.019"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/jes.2009.15"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2020.01.077"},{"key":"e_1_2_10_8_1","unstructured":"EMEA. 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