{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T11:31:46Z","timestamp":1769081506710,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T00:00:00Z","timestamp":1673481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An efficient method was established by high\u2010speed countercurrent chromatography (HSCCC) for the separation and purification of three flavonoids from <jats:italic>Oroxylum indicum<\/jats:italic>. Optimized by single\u2010factor and orthogonal experiments, the optimal extraction conditions were an extraction temperature of 50\u00b0C, a solid\u2010to\u2010liquid ratio of 1:50 (g\/ml), an ethanol concentration of 75% and an extraction time of 45\u2009min. Using a two\u2010phase solvent system composed of chloroform\u2013methanol\u2013water (6:10:5, v\/v\/v), the preparative separation was successfully performed by HSCCC in head\u2010to\u2010tail elution mode. Totals of 12.63\u2009mg of oroxin A at a purity of 97.61% with 96.46% recovery, 10.96\u2009mg oroxin B at a purity of 98.32% with 98.81% recovery, and 9.34\u2009mg baicalein at a purity of 98.64% with 97.87% recovery were obtained in one\u2010step separation from 200\u2009mg crude extract. Their chemical structures were confirmed by melting points, HPLC, UV, FTIR, MS, <jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C NMR data. Furthermore, they were efficient scavengers of 1,1\u2010diphenyl\u20102\u2010picrylhydrazyl and hydroxyl free radicals in a concentration\u2010dependent manner.<\/jats:p>","DOI":"10.1002\/bmc.5579","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T06:33:50Z","timestamp":1672900430000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Application of preparative high\u2010speed countercurrent chromatography for the separation and purification of three flavonoids from <i>Oroxylum indicum<\/i>"],"prefix":"10.1002","volume":"37","author":[{"given":"Yan","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University  Qingdao P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hu","family":"Shan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Qingdao Agricultural University  Qingdao P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9458-4307","authenticated-orcid":false,"given":"Haitao","family":"L\u00fc","sequence":"additional","affiliation":[{"name":"College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University  Qingdao P. R. 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