{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T18:43:15Z","timestamp":1777056195300,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T00:00:00Z","timestamp":1676073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Analytical techniques must be sensitive, specific, and accurate to assess the active pharmaceutical ingredients in pharmaceutical dosage forms. The quality\u2010by\u2010design (QbD) application has proven to be a practical method for magnifying HPLC operations. This article discusses the successfully developed QbD\u2010based stability\u2010indicative LC method for evaluating acetaminophen, caffeine, and aspirin (ASP) in tablet dosage form. To achieve the necessary chromatographic separation, Milli\u2010Q water, methanol, and glacial acetic acid were employed in the following ratios: 63:35:2 (v\/v\/v) for mobile phase A and 18:80:2 (v\/v\/v) for mobile phase B. The flow rate, column temperature, and detecting wavelength were 1.0\u00a0ml\/min, 40\u00b0C, and 275\u2009nm, respectively, and an InertSustain C18 analytical column (150\u2009\u00d7\u20094.6\u00a0mm, 3\u00a0\u03bcm) was used. Linearity was between 10.0 and 150.0\u00a0\u03bcg\/ml for ASP and acetaminophen and between 2.6 and 39.0\u00a0\u03bcg\/ml for caffeine. The accuracy findings were more than 97%, and the correlation coefficient for all three components was found to be greater than 0.999. The validated HPLC method yielded reliable and accurate results. ASP was shown to be vulnerable to both acid and alkaline hydrolysis in the forced degradation study. The described method is capable of separating the degradants produced during stress testing and is regarded as stability indicating. The proposed method can be used for a wider range of other formulations with an appropriate diluent selection and sample preparation procedure optimization.<\/jats:p>","DOI":"10.1002\/bmc.5585","type":"journal-article","created":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T09:13:55Z","timestamp":1674551635000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["An effective and stability\u2010indicating method development and optimization utilizing the Box\u2013Behnken design for the simultaneous determination of acetaminophen, caffeine, and aspirin in tablet formulation"],"prefix":"10.1002","volume":"37","author":[{"given":"Parvateesam","family":"Yenda","sequence":"first","affiliation":[{"name":"Department of Chemistry, School of Science GITAM Deemed to Be University  Hyderabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5737-8528","authenticated-orcid":false,"given":"Naresh Kumar","family":"Katari","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Science GITAM Deemed to Be University  Hyderabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7094-9161","authenticated-orcid":false,"given":"Santhosh Kumar","family":"Ettaboina","sequence":"additional","affiliation":[{"name":"Department of Quality Control Aurex Laboratories LLC  East Windsor New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balasubramanian","family":"Satheesh","sequence":"additional","affiliation":[{"name":"Analytical Research and Development Slayback Pharma India LLP, Manjeera Trinity Corporate, JNTU  Hyderabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1171-5189","authenticated-orcid":false,"given":"Siva Krishna","family":"Muchakayala","sequence":"additional","affiliation":[{"name":"Analytical Research and Development, Catalent Pharma Solutions  1100 Enterprise Dr Winchester Kentucky 40391 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0390-6505","authenticated-orcid":false,"given":"Rambabu","family":"Gundla","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Science GITAM Deemed to Be University  Hyderabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,2,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jaoac\/90.1.82"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-815799-2.00001-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(02)00421-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2016-0127"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.25004\/IJPSDR.2016.080306"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0b013e3181898b6f"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11094-022-02610-5"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.52711\/2231-5675.2022.00013"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/00032719408001095"},{"key":"e_1_2_9_11_1","unstructured":"ICH Q1A (R2). 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