{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T22:30:06Z","timestamp":1778538606906,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T00:00:00Z","timestamp":1678924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011789","name":"Department of Science and Technology of Jilin Province","doi-asserted-by":"publisher","award":["YDZJ202201ZYTS230"],"award-info":[{"award-number":["YDZJ202201ZYTS230"]}],"id":[{"id":"10.13039\/501100011789","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Tribulus terrestris<\/jats:italic><\/jats:styled-content> L. fruit (TT) is a traditional Chinese herbal medicine used to treat ischemic stroke (IS). This study aimed to investigate the protective effect of TT extract, named TT15, on middle cerebral artery occlusion (MCAO) rats using metabolomics and molecular docking and find the targets of action and the material basis of TT15 against IS. The results of the infarct volume and neurological defect scores confirmed the efficacy of TT15. Serum metabolomics analysis using LC\u2013MS revealed that model group animals experienced a variety of metabolic disturbances when compared to the sham group. TT15 can restore the MCAO\u2010induced serum metabolite changes by modulating multiple metabolic pathways. Six enzymes were highlighted by the metabolite\u2010reaction\u2010enzyme\u2010gene (M\u2010R\u2010E\u2010G) network analysis, which might be the possible targets for the TT15 against IS. Molecular docking analysis was applied to show the binding affinities between active compounds and these enzymes. The representative docking mode with the lowest binding energy between three compounds and phospholipase A 2 (PLA2) and peroxidase (POD) was displayed by the ribbon binding map. This study profiles the metabolic changes in MCAO\u2010induced IS and investigates the efficacy and the corresponding mechanism of TT15 in the treatment of IS.<\/jats:p>","DOI":"10.1002\/bmc.5614","type":"journal-article","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T01:49:46Z","timestamp":1678240186000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Investigating the <i>in vivo<\/i> effect of <scp><i>Tribulus terrestris<\/i><\/scp> extract in middle cerebral artery occlusion rats using LC\u2013MS\u2010based metabolomics combined with molecular docking"],"prefix":"10.1002","volume":"37","author":[{"given":"Xingxing","family":"Li","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Yanbian University  Yanji China"},{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjun","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Yanbian University  Yanji China"},{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohang","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqing","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingzi","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Yanbian University  Yanji China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2582-7480","authenticated-orcid":false,"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-020-00685-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201802842RR"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2021.04.010"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26165001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq048"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113202"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S220314"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2MO00216G"},{"issue":"35","key":"e_1_2_8_10_1","first-page":"156","article-title":"Research progress of Tribulus terrestris composition and main pharmacologic effects","volume":"11","author":"Hou S.","year":"2014","journal-title":"China Medical Herald"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2005.09.020"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.618796"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2011.04.009"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2015.10.086"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.12950"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.24612"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/18715273112119990053"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.25541"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10010065"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10068-017-0165-4"},{"issue":"4","key":"e_1_2_8_21_1","first-page":"223","article-title":"Research progress on pharmacological action of caltrop","volume":"32","author":"Li B. L.","year":"2011","journal-title":"Journal of Jilin Medical College"},{"issue":"4","key":"e_1_2_8_22_1","first-page":"341","article-title":"Expression and significance of homocysteine and glutathione peroxidase in acute ischemic stroke","volume":"27","author":"Li J.","year":"2020","journal-title":"Pharmaceutical Biotechnology"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/13102818.2014.901678"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.5582\/bst.2017.01046"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-021-03918-3"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab382"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2337\/dci19-0037"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13875"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.13194\/j.jlunivtcm.2007.03.184.qunn.123"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2019.01.010"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants9121764"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2012-202033"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5RA14058G"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24040793"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.12.061"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1062"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2022.104297"},{"issue":"4","key":"e_1_2_8_39_1","first-page":"1594","article-title":"Research progress on chemical constituents and metabolism in vitro and in vivo of Fructus Tribuli","volume":"34","author":"Xu D. D.","year":"2019","journal-title":"China Journal of Traditional Chinese Medicine Pharmacy"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.00887"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000005803"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5614","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.5614","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5614","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:56:39Z","timestamp":1760046999000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5614"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,16]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1002\/bmc.5614"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5614","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,16]]},"assertion":[{"value":"2022-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5614"}}