{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:09:29Z","timestamp":1760141369890,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T00:00:00Z","timestamp":1682985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Valproate and lamotrigine are commonly used as antiepileptic drugs even in pregnant and breastfeeding women. The extent and effects of drug exposure on the developing brain of the offspring are not well understood. Animal models can be utilised to investigate the transfer of substances into fetal brain with the ultimate aim of providing insights to aid clinical decisions. In the present study, an LC\u2013MS\/MS method was developed and validated for quantification of valproate (VPA), valproate\u2010glucuronide (VPA\u2010Gluc, a major metabolite of valproate) and lamotrigine (LTG) in rat blood plasma, cerebrospinal fluid and brain tissue. A 10 \u03bcl sample was spiked with stable isotope\u2010labelled internal standards and extracted by methanol. An Agilent RRHD Eclipse Plus C<jats:sub>18<\/jats:sub> column (2.1\u2009\u00d7\u2009100\u2009mm, 1.8\u2009\u03bcm) was used. The MS\/MS transitions were 143.1016\u2013143.1016 (VPA), 319.1392\u2013143.0978 (VPA\u2010Gluc) and 256.0157\u2013210.9826 (LTG). The linear ranges of VPA, VPA\u2010Gluc and LTG were 30\u2013250, 10\u2013140 and 0.3\u20131\u2009\u03bcg\/ml, respectively. The intra\u2010 and inter\u2010day accuracy and precision, carryover, sensitivity and recovery were evaluated according to the US Food and Drug Administration guidance for bioanalytical method validation. Finally, the validated method was applied to a set of experimental animal samples and produced results highly comparable with those from an orthogonal analytical method.<\/jats:p>","DOI":"10.1002\/bmc.5652","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T01:14:49Z","timestamp":1681434889000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Liquid chromatography coupled with tandem mass spectrometry for simultaneous quantification of valproate, valproate\u2010glucuronide and lamotrigine in various biological matrices of rat"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0642-1116","authenticated-orcid":false,"given":"Fiona","family":"Qiu","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Central Clinical School Monash University  Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Nie","sequence":"additional","affiliation":[{"name":"Melbourne Mass Spectrometry and Proteomics Facility, Bio 21 Molecular Science and Biotechnology Institute University of Melbourne  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2003.029132"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semperi.2015.08.003"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.05.022"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/FPC.0b013e32835ea0b2"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1692"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/npr2.12045"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2006.09.033"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-200306000-00016"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.20078.1"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2015.11.006"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60461-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-200210000-00009"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1528-1157.2002.13001.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2165\/00023210-200216100-00004"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2021.06.005"},{"key":"e_1_2_9_17_1","unstructured":"Qiu F. Huang Y. Habgood M. D. Dziegielewska K. M. &Saunders N.(2023 in preparation).Brain and placental entry of valproate and lamotrigine in a rat model of epilepsy (GAERS)."},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.52607.1"},{"key":"e_1_2_9_19_1","unstructured":"US Department of Health and Human Services. (2018).Bioanalytical method validation guidance for industry.https:\/\/www.fda.gov\/files\/drugs\/published\/Bioanalytical-Method-Validation-Guidance-for-Industry.pdf"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seizure.2010.07.019"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eplepsyres.2014.04.005"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000123"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.1992.tb04079.x"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5652","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:52:09Z","timestamp":1760057529000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5652"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,2]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1002\/bmc.5652"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5652","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2023,5,2]]},"assertion":[{"value":"2023-01-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5652"}}