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We aimed to explore the changes in fecal metabolism after CT\u2010guided RFA in CC mice. The orthotopic CC mice received CT\u2010guided RFA upon modeling. Subsequently, we quantified tumor volumes and weights to assess treatment efficacy. Next, because metabolomics is useful for evaluating therapeutic validity, feces were collected for metabolomics analysis. CT\u2010guided RFA inhibited tumor growth effectively. Additionally, metabolomics results showed that the contents of bile acids and fatty acids were downregulated in CC mouse feces. Moreover, the levels of amino acids and carbohydrates were decreased while the levels of fatty acids, organic acids, phenols, pyridines and short\u2010chain fatty acids were elevated in feces after CC mice received CT\u2010guided RFA. Pathway enrichment analysis revealed that those differential metabolites were closely related to fatty acids degradation and synthesis. CT\u2010guided RFA possesses a strong ability to suppress CC development in mice, accompanied by a significant increase of fatty acid content in feces. This study proposes a novel approach and target for CC treatment, which provides hope for CC patients and establishes a solid basis for future in\u2010depth studies.<\/jats:p>","DOI":"10.1002\/bmc.5658","type":"journal-article","created":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T21:58:50Z","timestamp":1682027930000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Ultra\u2010high\u2010performance liquid chromatography\u2010tandem mass spectrometry\u2010based metabolomics unveils the metabolic alterations in colon cancer mice during CT\u2010guided radiofrequency ablation"],"prefix":"10.1002","volume":"37","author":[{"given":"Jin","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Special Inspection The Third Affiliated Hospital of Zhejiang Chinese Medical University  Hangzhou 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