{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T03:26:29Z","timestamp":1769829989839,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,9,6]],"date-time":"2005-09-06T00:00:00Z","timestamp":1125964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A reliable and validated reversed\u2010phase high\u2010performance liquid chromatography (HPLC) method using fluorescence detection is reported for the simultaneous quantitation of mycophenolic acid (MPA) and valproic acid (VPA) in human plasma. The method is based on the pre\u2010column derivatization of valproic acid with 4\u2010bromomethyl\u20106, 7\u2010dimethoxycoumarin (BrMMC) and online solvatochromism of MPA by pH adjustment. The linear calibration range was 0.50\u201330 \u00b5g\/mL for MPA and 5.00\u2013150 \u00b5g\/mL for VPA. The relative standard deviations of the method of intra\u2010 and inter\u2010day analyses (<jats:italic>n<\/jats:italic> = 6) were below 6.5 and 6.7% for MPA, and 5.8 and 6.3% for VPA, respectively. Dichloromethane was used for the simultaneous extraction of MPA and VPA from acidified plasma. This reliable method can be applied in the analysis of MPA and VPA in human plasma using only a small volume (100 \u00b5L). Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.566","type":"journal-article","created":{"date-parts":[[2005,9,6]],"date-time":"2005-09-06T12:46:55Z","timestamp":1126010815000},"page":"319-326","source":"Crossref","is-referenced-by-count":25,"title":["Simultaneous determination of mycophenolic acid and valproic acid based on derivatization by high\u2010performance liquid chromatography with fluorescence detection"],"prefix":"10.1002","volume":"20","author":[{"given":"Yan","family":"Zhong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunqiu","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,9,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)80166-8"},{"key":"e_1_2_1_3_1","first-page":"257","article-title":"Clean\u2010up and determination of sodium valproate in serum by high\u2010performance liquid chromatography with fluorometric detection","volume":"46","author":"Bousquet E","year":"1991","journal-title":"Pharmazie"},{"key":"e_1_2_1_4_1","first-page":"296","article-title":"Determination of valproic acid in serum by high performance liquid chromatography","volume":"9","author":"Chen J","year":"2000","journal-title":"Chinese Journal of Clinical Pharmacy"},{"key":"e_1_2_1_5_1","first-page":"458","article-title":"Determination of valproic acid in serum by high performance liquid chromatography","volume":"21","author":"Chen ZF","year":"2001","journal-title":"Chinese Journal of Hospital Pharmacy"},{"key":"e_1_2_1_6_1","unstructured":"1995 Appleton and Lange Connecticut WR Garnett GE Schumacher Antiepileptic drugs 357"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00498-8"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(00)00442-8"},{"key":"e_1_2_1_9_1","first-page":"355","article-title":"Simultaneous determination of mycophenolic acid and its glucuronide conjugate in human plasma by a single\u2010run ion paring method","volume":"24","author":"Huang JZ","year":"2001","journal-title":"Journal of Pharmaceutical and Biomedical Analysis"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(97)00678-6"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.07.025"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(92)80189-W"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1093\/clinchem\/34.1.141","article-title":"Automated determination of drugs in serum by liquid chromatography with column\u2010switching I. 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