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Amentoflavone was successfully encapsulated into the core, achieving an encapsulation efficiency of 98.80\u2009\u00b1\u20090.24% and a drug loading efficiency of 2.96\u2009\u00b1\u20090.12%. The resulting micelles exhibited a spherical shape with a particle size of approximately 25.99\u2009nm. The solubility of AF was significant improved by 412\u2010fold, and cumulative drug release studies showed that AF release was much faster from the micelles compared with the free drug. The release of AF was sustained over time and followed a degradation\u2010based kinetic model, similar to polymeric systems. After oral administration, the AF\u2010loaded micelles demonstrated an enhanced oral bioavailability, which was 3.79 times higher than that of free AF. <jats:italic>In vitro<\/jats:italic> evaluations of the micelles\u2019 antitumor effects revealed a significantly greater efficacy compared with free AF. These findings highlight the tremendous potential of DSPE\u2010PEG<jats:sub>2000<\/jats:sub> micelles as a drug delivery carrier for improving the solubility and therapeutic efficacy of AF.<\/jats:p>","DOI":"10.1002\/bmc.5690","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T00:10:22Z","timestamp":1687219822000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Preparation of amentoflavone\u2010loaded DSPE\u2010PEG<sub>2000<\/sub> micelles with improved bioavailability and <i>in vitro<\/i> antitumor efficacy"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3707-7391","authenticated-orcid":false,"given":"Yuan","family":"Feng","sequence":"first","affiliation":[{"name":"Hebei Key Laboratory of Neuropharmacology Hebei North University  Zhangjiakou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4798-3465","authenticated-orcid":false,"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Neuropharmacology Hebei North University  Zhangjiakou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaishuai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Li","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Neuropharmacology Hebei North University  Zhangjiakou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Neuropharmacology Hebei North University  Zhangjiakou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Neuropharmacology Hebei North University  Zhangjiakou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingzhe","family":"Qiu","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Neuropharmacology Hebei North University  Zhangjiakou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8454-0674","authenticated-orcid":false,"given":"Yuanyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Neuropharmacology Hebei North University  Zhangjiakou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.07.159"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26165052"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2015.12.031"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.10.028"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.14893"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2017.09.005"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S146315"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultramic.2017.07.001"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2005.09.034"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2021.02.031"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S150366"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2016.10.005"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.03.010"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl048037v"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf5019615"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S51566"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2014.991321"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612826666200526150113"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2011.08.067"},{"issue":"6","key":"e_1_2_8_21_1","first-page":"963","article-title":"Amentoflavone induces cell\u2010cycle arrest and apoptosis in MCF\u20107 human breast cancer cells via mitochondria\u2010dependent pathway","volume":"26","author":"Pei J. 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