{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:54:33Z","timestamp":1760061273300,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Anoectochilus roxburghii<\/jats:italic> (Wall.) Lindl. (AR) has been traditionally used to treat inflammatory diseases, but the specific mechanism underlying its hepatoprotective effect remains unclear. Here, serum metabolomics and network pharmacology were employed to investigate the hepatoprotective mechanism of AR. Thirty male Sprague\u2013Dawley rats were divided into six groups: normal, model, positive, high\u2010dose AR, middle\u2010dose AR, and low\u2010dose AR. The positive group received therapeutic doses of silibinin, whereas the AR\u2010treated groups received different doses of AR extract once daily. After 10\u2009days of intragastric administration, the rats were intraperitoneally injected with a 50% CCl<jats:sub>4<\/jats:sub> olive oil solution (2\u2009mL\/kg) to induce liver injury. Serum and liver samples were obtained, and GC\u2013MS was utilized to monitor changes in serum metabolome. The levels of alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, and hydrooxproline in serum significantly increased in the model group. On the contrary, AR\u2010treated group showed a significant decrease in the levels of alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, and hydrooxproline. Histopathological observation also revealed that the extent of liver injury was alleviated in the AR\u2010treated group. Fifty differential metabolites were identified, suggesting that AR may prevent liver damage by modulating carbohydrate and amino acid metabolism.<\/jats:p>","DOI":"10.1002\/bmc.5706","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:57:39Z","timestamp":1690333059000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Serum metabolome profiling, network pharmacology analysis, and experimental validation of <i>Anoectochilus roxburghii<\/i> in the treatment of carbon tetrachloride\u2013induced liver injury"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6838-0749","authenticated-orcid":false,"given":"Yi","family":"Tao","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou 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