{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:54:53Z","timestamp":1760061293904,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T00:00:00Z","timestamp":1692662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dolutegravir (DTG) has been the first\u2010line drug in many human immunodeficiency virus\/acquired immunodeficiency syndrome (HIV\/AIDS) guidelines for the treatment of na\u00efve and experienced HIV\u2010infected individuals, which calls for cost\u2010effective and convenient methods for quantitative detection of DTG in human plasma for pharmacokinetic studies and patient adherence evaluation. Here, an HPLC\u2013ultraviolet method in combination with liquid\u2013liquid extraction with isocratic elution was developed for the first time. The analysis was performed on a CLC\u2010ODS column (6\u2009mm internal diameter \u00d7\u200915\u2009cm, 5\u2009\u03bcm) using a mixture of acetonitrile and phosphate buffer (40:60, <jats:italic>v\/v<\/jats:italic>) as the mobile phase at the flow rate of 1\u2009mL\/min. Using triamcinolone as the internal standard, 100\u2009\u03bcL of plasma sample was extracted by methyl tert\u2010butyl ether, followed by evaporating under nitrogen stream, re\u2010dissolving with 100\u2009\u03bcL mobile phase, and injection of 20\u201340\u2009\u03bcL of supernatant into the chromatographic system. The linearity of DTG was good in the range of 0.05\u201310\u2009\u03bcg\/mL (<jats:italic>r<\/jats:italic>\u00a0=\u20090.9995), and the inter\u2010 and intra\u2010day variabilities were 0.4%\u20134.3% (<jats:italic>n<\/jats:italic>\u00a0=\u200910) and 1.2%\u20136.2% (<jats:italic>n<\/jats:italic>\u00a0=\u200910) for the lower limit of quantification, low\u2010, medium\u2010, and high\u2010concentration quality control samples (0.05, 0.1, 0.8, and 8\u2009\u03bcg\/mL), respectively, while the methodological and extraction recoveries were 98.0%\u2013103.0% (<jats:italic>n<\/jats:italic>\u00a0=\u200920) and 65.2%\u201375.7% (<jats:italic>n<\/jats:italic>\u00a0=\u20093), respectively. This method was successfully applied to analyze DTG plasma concentration in 84 Chinese patients with HIV.<\/jats:p>","DOI":"10.1002\/bmc.5708","type":"journal-article","created":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T03:05:23Z","timestamp":1692673523000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development and validation of an HPLC method for quantification of dolutegravir in human plasma"],"prefix":"10.1002","volume":"37","author":[{"given":"Dongsheng","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking 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