{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:08:35Z","timestamp":1790780915203,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["Y21H280036"],"award-info":[{"award-number":["Y21H280036"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014717","name":"National Outstanding Youth Science Fund Project of National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703701"],"award-info":[{"award-number":["81703701"]}],"id":[{"id":"10.13039\/100014717","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Achyranthes bidentata<\/jats:italic><\/jats:styled-content> has been found to possess beneficial effects against osteoporosis, but there is still a lack of comprehensive studies on its anti\u2010osteoporotic compounds. Therefore, in this study, we established a zebrafish osteoporosis model to evaluate the anti\u2010osteoporotic effect of different fractions of raw and salt\u2010processed <jats:styled-content style=\"fixed-case\"><jats:italic>A. bidentata<\/jats:italic><\/jats:styled-content>. Among these fractions, the dichloromethane fraction showed the most promising anti\u2010osteoporotic effect. To further investigate the active compounds responsible for the anti\u2010osteoporosis effects, we prepared and analyzed the dichloromethane fraction of 10 batches of raw and salt\u2010processed <jats:styled-content style=\"fixed-case\"><jats:italic>A. bidentata<\/jats:italic><\/jats:styled-content> using liquid chromatography\u2013mass spectrometry. As a result, we tentatively identified 19 compounds, including 11 saponins, three phenolic amides, three unsaturated fatty acids and two other compounds. To further narrow down the potential active compounds, we employed both orthogonal partial least squares discriminant analysis and gray relationship analysis. Through these analyses, we were able to identify eight compounds that showed a high correlation with the anti\u2010osteoporosis effects of the dichloromethane fraction. Furthermore, we validated the anti\u2010osteoporotic effects of <jats:italic>\u03b2<\/jats:italic>\u2010ecdysterone, wogonin, ginsenoside Ro, oleanolic acid, linoleic acid and palmitic acid using the zebrafish model. These compounds demonstrated significant anti\u2010osteoporotic effects, further supporting their potential as active compounds in <jats:styled-content style=\"fixed-case\"><jats:italic>A. bidentata<\/jats:italic><\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/bmc.5742","type":"journal-article","created":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T03:40:47Z","timestamp":1694058047000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Anti\u2010osteoporosis bioactivity evaluation in zebrafish model of raw and salt\u2010processed <scp><i>Achyranthes bidentata<\/i><\/scp> followed by liquid chromatography\u2013mass spectrometry analysis and correlation analysis"],"prefix":"10.1002","volume":"37","author":[{"given":"Ying","family":"Yang","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisha","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6838-0749","authenticated-orcid":false,"given":"Yi","family":"Tao","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00006"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123265"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32112-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201700575"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-017-0205-1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.03.035"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-019-01355-y"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.04.027"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/102860203100001625094"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2022.2053851"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-021-00210-8"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2023.105525"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202100272"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2022.115078"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(12)60003-X"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.54.676"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201601447"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imr.2015.04.238"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.5742","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:08:38Z","timestamp":1760040518000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.5742"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,7]]},"references-count":18,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1002\/bmc.5742"],"URL":"https:\/\/doi.org\/10.1002\/bmc.5742","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,7]]},"assertion":[{"value":"2023-07-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5742"}}