{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T02:38:55Z","timestamp":1767494335117,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pharmacology experts place a high priority on therapeutic plants because the majority of pharmaceutical firms rely on medicinal plants as raw ingredients. Therefore, the potential bioactive components using gas chromatography\u2013mass spectrometry analysis and antioxidant effects using DPPH free radical scavenging activity of various crude fractions of <jats:styled-content style=\"fixed-case\"><jats:italic>Xanthium spinosum<\/jats:italic><\/jats:styled-content> were assessed. Gas chromatography\u2013mass spectrometry analysis showed the presence of various bioactive compounds including benzenedicarboxylic acid (18.60%), 8\u2010octadecenoic acid (4.86%), 11\u2010octadecenoic acid and 10\u2010octadecenoic acid in the crude methanolic extract, 1,2\u2010benzenedicarboxylic acid, diisooctyl ester (14.42%), 1,2\u2010benzenedicarboxylic acid, mono (2\u2010ethylhexyl) ester (14.42%), 6\u2010octadecenoic acid, methyl ester (7.56%), 8\u2010octadecenoic acid, methyl ester (7.56%), 10\u2010octadecenoic acid, methyl ester (7.56%) and hexadecanoic acid, methyl ester (6.55%) in the <jats:italic>n<\/jats:italic>\u2010hexane extract, ethanal, 2\u2010methyl\u20102\u2010[4\u2010(1\u2010methylethyl)phenyl]\u2010(3.02%), (+)\u20103\u2010carene, 4\u2010isopropenyl\u2010(3.02%), 7H\u2010indeno[5,6\u2010b] furan\u20107\u2010one, 4,4a,5,6,7a,8\u2010hexahydro\u2010 (3.02%) and 2\u2010[5\u2010(2,2\u2010dimethyl\u20106\u2010methylene\u2010cyclohexyl)\u20103\u2010methyl\u2010pent\u20102\u2010enyl]\u2010[1,4] benzoquinone (2.79%) in the chloroform extract and 1,2\u2010benzenedicarboxylic acid, mono (2\u2010ethylhexyl) ester (33.005%), 1,2\u2010benzenedicarboxylic acid, diisooctyl ester (33.005%) and bis(2\u2010ethylhexyl) phthalate (33.005%) in the ethyl acetate extract. Significant DPPH radical scavenging activity was exhibited by the chloroform fraction (43.37\u201388.65%) at all doses followed by the crude methanolic extract (36.02\u201383.75%) at all doses. In conclusion, different crude fractions of <jats:styled-content style=\"fixed-case\"><jats:italic>X. spinosum<\/jats:italic><\/jats:styled-content> can be considered a rich source of pharmacologically active components that can be scoped for isolation and may be subjected to in\u2010depth pharmacological study.<\/jats:p>","DOI":"10.1002\/bmc.5776","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T18:00:29Z","timestamp":1700589629000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Chromatographic analysis and antioxidant potency of the crude extract of <scp><i>Xanthium spinosum<\/i><\/scp> in various fractions"],"prefix":"10.1002","volume":"38","author":[{"family":"Barkatullah","sequence":"first","affiliation":[{"name":"Pharmacognosy Laboratory, Department of Botany Islamia College Peshawar  Peshawar Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Shabana","sequence":"additional","affiliation":[{"name":"Pharmacognosy Laboratory, Department of Botany Islamia College Peshawar  Peshawar Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9458-3873","authenticated-orcid":false,"given":"Muhammad","family":"Nafees","sequence":"additional","affiliation":[{"name":"Pharmacognosy Laboratory, Department of Botany University of Peshawar  Peshawar Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,20]]},"reference":[{"issue":"6","key":"e_1_2_10_2_1","first-page":"22","article-title":"GC\u2010MS and FT\u2010IR analysis of constituents of essential oil from cinnamon bark growing in South\u2010west of Ethiopia","volume":"1","author":"Adinew B.","year":"2014","journal-title":"International Journal of Herbal Medicine"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.24925\/turjaf.v10i2.272-275.4685"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2013.07.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2013.09.015"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27041326"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.17957\/IJAB\/15.1727"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.5958\/0974-360X.2016.00043.3"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-87604-1"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.4489\/MYCO.2009.37.1.031"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations9090244"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2017.06.032"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2021.06.023"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.5958\/0975-6892.2022.00008.9"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjbas.2018.02.002"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants9030331"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/2210315510999200527142244"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.937946"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.23924"},{"issue":"5","key":"e_1_2_10_20_1","first-page":"1236","article-title":"Pharmacognostic and phytochemical screening of Cocculus pendulus Diels. stem and root","volume":"30","author":"Nafees M.","year":"2020","journal-title":"Journal of Animal & Plant Sciences"},{"issue":"1","key":"e_1_2_10_21_1","first-page":"216","article-title":"GC\u2010MS analysis of phytocomponents in Pleiospermium alatum (Wall. ex Wight & Arn.) 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