{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:34:35Z","timestamp":1786977275317,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T00:00:00Z","timestamp":1700611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012542","name":"Sichuan Province Science and Technology Support Program","doi-asserted-by":"publisher","award":["2023YF0047"],"award-info":[{"award-number":["2023YF0047"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014717","name":"National Outstanding Youth Science Fund Project of National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81903722"],"award-info":[{"award-number":["81903722"]}],"id":[{"id":"10.13039\/100014717","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Although levetiracetam (LEV) has favorable linear pharmacokinetic properties, therapeutic drug monitoring (TDM) is necessary for pregnant women with epilepsy. This study aims to build a simple, reliable, and sensitive ultra\u2010performance liquid chromatography\u2013tandem mass spectrometry (UPLC\u2013MS\/MS) method for determining LEV concentrations in plasma and saliva samples, to support the routine TDM of LEV in Chinese pregnant women with epilepsy. The stable isotope\u2010labeled LEV\u2010d\n                    <jats:sub>6<\/jats:sub>\n                    was used as the internal standard. The extracted samples were analyzed using a UPLC\u2013MS\/MS system with positive electrospray ionization. Mobile phase A was water containing 5\u2009mM ammonium acetate and 0.1% formic acid, and phase B was 1:1 methanol\u2013acetonitrile with 0.1% formic acid. The method was validated and utilized to determine LEV concentrations in non\u2010pregnant and pregnant patients with epilepsy. The developed method was validated in both plasma and saliva samples over a concentration range of 0.1\u201350\u2009\u03bcg\/mL. The intra\u2010 and inter\u2010batch accuracy for LEV ranged from \u22127.0% to 2.9%, with precisions between 2.7% and 9.3%. In pregnant patients, the mean dose\u2010standardized LEV trough plasma concentrations were significantly lower than those in non\u2010pregnant patients (4.73\u2009\u00b1\u20092.99 vs. 7.74\u2009\u00b1\u20093.59\u2009ng\/mL per mg\/day;\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.0001). It is recommended that the TDM of LEV should be routinely performed during the different stages of pregnancy.\n                  <\/jats:p>","DOI":"10.1002\/bmc.5777","type":"journal-article","created":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T19:00:16Z","timestamp":1700766016000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Quantitation of levetiracetam concentrations in plasma and saliva samples by ultra\u2010performance liquid chromatography\u2013tandem mass spectrometry: Application to therapeutic drug monitoring for pregnant women with epilepsy"],"prefix":"10.1002","volume":"38","author":[{"given":"Mengyu","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Neurology West China Hospital of Sichuan University  Chengdu Sichuan Province China"},{"name":"Department of Pharmacy NMPA Key Laboratory for Clinical Research and Evaluation of Innovative Drug West China Hospital of Sichuan University  Chengdu Sichuan Province China"},{"name":"Clinical Trial Center West China Hospital of Sichuan University  Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Neurology West China Hospital of Sichuan University  Chengdu Sichuan Province China"},{"name":"Department of Pharmacy NMPA Key Laboratory for Clinical Research and Evaluation of Innovative Drug West China Hospital of Sichuan University  Chengdu Sichuan Province China"},{"name":"Clinical Trial Center West China Hospital of Sichuan University  Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanling","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology West China Hospital of Sichuan University  Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoqun","family":"He","sequence":"additional","affiliation":[{"name":"Department of Neurology West China Hospital of Sichuan University  Chengdu Sichuan Province China"},{"name":"Department of Pharmacy NMPA Key Laboratory for Clinical Research and Evaluation of Innovative Drug West China Hospital of Sichuan University  Chengdu Sichuan Province China"},{"name":"Clinical Trial Center West China Hospital of Sichuan University  Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangjie","family":"Di","sequence":"additional","affiliation":[{"name":"Department of Neurology West China Hospital of Sichuan University  Chengdu Sichuan Province China"},{"name":"Department of Pharmacy NMPA Key Laboratory for Clinical Research and Evaluation of Innovative Drug West China Hospital of Sichuan University  Chengdu Sichuan Province China"},{"name":"Clinical Trial Center West China Hospital of Sichuan University  Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Neurology West China Hospital of Sichuan University  Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurology West China Hospital of Sichuan University  Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenlei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology West China Hospital of Sichuan University  Chengdu Sichuan Province China"},{"name":"Department of Pharmacy NMPA Key Laboratory for Clinical Research and Evaluation of Innovative Drug West China Hospital of Sichuan University  Chengdu Sichuan Province China"},{"name":"Clinical Trial Center West China Hospital of Sichuan University  Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2021.113961"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bky064"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2022.106349"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ackd.2013.01.012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00023-5"},{"key":"e_1_2_7_7_1","unstructured":"EMA. 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