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We propose a novel method for quantifying HbA and HbS using the glycated haemoglobin (HbA1c) assay on a Tosoh HLC\u2010723G8 (G8) analyser in variant mode. HbA and HbS results obtained using our method highly correlated with results obtained using a reference method (<jats:italic>r<\/jats:italic>\u2009&gt;\u20090.99 for 124 samples of patients with SCD or sickle cell trait). Our method met laboratory requirements for linearity (coefficient of variation [CV] and bias &lt;5%), between\u2010run and within\u2010run reproducibility (CV &lt;10%) and carryover (&lt;0.5%) over the range of HbS and HbA values expected in a therapeutic context. Using the G8 analyser in variant mode is viable for monitoring HbA and HbS concentrations in dire situations. This method is easy to use, quick (1.6\u00a0min per sample), and automatable and produces highly reproducible results without significant bias. Finally, it does not require modifications to the analytical pipeline recommended by the supplier, enabling a 24\u20107 availability without disrupting routine monitoring of HbA1c in the laboratory.<\/jats:p>","DOI":"10.1002\/bmc.5799","type":"journal-article","created":{"date-parts":[[2023,12,2]],"date-time":"2023-12-02T11:04:09Z","timestamp":1701515049000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rapid haemoglobin A and S quantification using Tosoh HLC\u2010723G8 in variant mode for patients with sickle cell disease"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7717-3397","authenticated-orcid":false,"given":"Julien","family":"Fadel","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Biology University Hospital of Angers  Angers 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Inserm U1083, CNRS 6015 University of Angers  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Manuel Chao","family":"de la Barca","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Biology University Hospital of Angers  Angers France"},{"name":"Mitolab, Mitovasc, Mixed Research Unit (UMR) Inserm U1083, CNRS 6015 University of Angers  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascal","family":"Reynier","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Biology University Hospital of Angers  Angers France"},{"name":"Mitolab, Mitovasc, Mixed Research Unit (UMR) Inserm U1083, CNRS 6015 University of Angers  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coralie","family":"Mallebranche","sequence":"additional","affiliation":[{"name":"Department of Paediatric Haematology, Oncology and Immunology University 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