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We also analyzed the pharmacokinetics of those components after oral administration of fried Radix Paeoniae Alba (FRPA) in beagle dogs. Plasma samples were processed by protein precipitation with methanol. Chromatographic separation was performed with a Waters HSS\u2010T3 C<jats:sub>18<\/jats:sub> column (100\u2009\u00d7\u20092.1\u00a0mm, 1.8\u00a0\u03bcm, kept at 40\u00b0C) using multiple reaction monitoring mode. A gradient elution procedure was used with solvent A (0.02% formic acid\u2013water) and solvent B (0.02% formic acid\u2013acetonitrile) as mobile phases. Method validation was performed as US Food and Drug Administration guidelines, and the results met the acceptance criteria. The method we establish in this experiment was successfully applied to the pharmacokinetic study after oral administration of FRPA extract to beagle dogs.<\/jats:p>","DOI":"10.1002\/bmc.5803","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T02:15:58Z","timestamp":1702606558000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Simultaneous determination of five compounds of fried Radix Paeoniae Alba extract in beagle dogs plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry and its application in a pharmacokinetic study"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4959-9355","authenticated-orcid":false,"given":"Wenhao","family":"Cheng","sequence":"first","affiliation":[{"name":"Center for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"},{"name":"School of Chinese Pharmacy Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7589-6727","authenticated-orcid":false,"given":"Zheng","family":"Yuan","sequence":"additional","affiliation":[{"name":"Center for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siyang","family":"Wu","sequence":"additional","affiliation":[{"name":"Center for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Yu","sequence":"additional","affiliation":[{"name":"Center for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kexin","family":"Xia","sequence":"additional","affiliation":[{"name":"Center for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lifeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuyan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Chinese Medicine Shandong University of Traditional Chinese Medicine  Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Kang","sequence":"additional","affiliation":[{"name":"Center for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luyang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biology Zhongkai University of Agriculture and Engineering  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4201-1803","authenticated-orcid":false,"given":"Yingfei","family":"Li","sequence":"additional","affiliation":[{"name":"Center for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.630970"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16119600"},{"key":"e_1_2_8_4_1","first-page":"108","volume-title":"Pharmacopoeia of the People\u2019s Republic of China, Vol. \u0399","author":"Chinese Pharmacopoeia Commission","year":"2020"},{"key":"e_1_2_8_5_1","unstructured":"FDA. 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