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However, the lipidomics and metabolics bases of childhood <jats:styled-content style=\"fixed-case\"><jats:italic>M. pneumoniae<\/jats:italic><\/jats:styled-content> infections remain unclear. In this study, lipidomics and metabolomics analyses were conducted using UHPLC\u2010LTQ\u2010Orbitrap XL mass spectrometry and gas chromatography\u2010triple quadrupole mass spectrometry on plasma (<jats:italic>n<\/jats:italic>\u2009=\u200965) and urine (<jats:italic>n<\/jats:italic>\u2009=\u200965) samples. MS\u2010DIAL software, in combination with LipidBlast and Fiehn BinBase DB, identified 163 lipids and 104 metabolites in plasma samples, as well as 208 metabolites in urine samples. Perturbed lipid species (adjusted <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) were observed, including lysophosphatidylethanolamines, phosphatidylinositols, phosphatidylcholines, phosphatidylethanol amines, and triglycerides. Additionally, differential metabolites (adjusted <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) exhibited associations with amino acid metabolism, nucleotide metabolism, and energy metabolism. Thirteen plasma metabolites, namely <jats:sc>l<\/jats:sc>\u2010hydroxyproline, 3\u2010phosphoglycerate, citric acid, creatine, inosine, ribitol, \u03b1 tocopherol, cholesterol, cystine, serine, uric acid, tagatose, and glycine, showed significant associations with disease severity (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and exhibited distinct separation patterns in <jats:styled-content style=\"fixed-case\"><jats:italic>M. pneumoniae\u2013<\/jats:italic><\/jats:styled-content>infected bronchitis and pneumonia, with an area under the curve of 0.927. Nine of them exhibited either positive or negative correlations with neutrophil or lymphocyte percentages. These findings indicated significant systemic metabolic shifts in childhood <jats:styled-content style=\"fixed-case\"><jats:italic>M. pneumoniae<\/jats:italic><\/jats:styled-content> infections, offering valuable insights into the associated metabolic alterations and their relationship with disease severity.<\/jats:p>","DOI":"10.1002\/bmc.5817","type":"journal-article","created":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T04:12:20Z","timestamp":1703218340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Integration of lipidomics and metabolomics reveals plasma and urinary profiles associated with pediatric <scp><i>Mycoplasma pneumoniae<\/i><\/scp> infections and its severity"],"prefix":"10.1002","volume":"38","author":[{"given":"Hui","family":"Chen","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Medical Metabolomics Center, Pediatrics Department Affiliated Hospital of Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010bin","family":"Chen","sequence":"additional","affiliation":[{"name":"The First Clinical Medical College Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010na","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Chinese Medicine Beijing Children's Hospital Affiliated to Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010xia","family":"Yuan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Medical Metabolomics Center, Pediatrics Department Affiliated Hospital of Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8079-7950","authenticated-orcid":false,"given":"Jin\u2010jun","family":"Shan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Medical Metabolomics Center, Pediatrics Department Affiliated Hospital of Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2051-3420","authenticated-orcid":false,"given":"Shou\u2010chuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Medical Metabolomics Center, Pediatrics Department Affiliated Hospital of Nanjing University of Chinese Medicine  Nanjing 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