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The metabolic profile of epileptogenesis is still poorly investigated. Elucidation of such a metabolic profile using animal models of epilepsy could help identify new metabolites and pathways involved in the mechanisms of epileptogenesis process. In this study, we evaluated the metabolic profile during the epileptogenesis periods. Using a pilocarpine model of epilepsy, we analyzed the global metabolic profile of hippocampal extracts by untargeted metabolomics based on ultra\u2010performance liquid chromatography\u2013high\u2010resolution mass spectrometry, at three time points (3\u00a0h, 1 week, and 2\u00a0weeks) after\n                    <jats:italic>status epilepticus<\/jats:italic>\n                    (\n                    <jats:italic>SE<\/jats:italic>\n                    ) induction. We demonstrated that epileptogenesis periods presented different hippocampal metabolic profiles, including alterations of metabolic pathways of amino acids and lipid metabolism. Six putative metabolites (tryptophan,\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetylornithine,\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetyl\u2010\n                    <jats:sc>L<\/jats:sc>\n                    \u2010aspartate, glutamine, adenosine, and cholesterol) showed significant different levels during epileptogenesis compared to their respective controls. These putative metabolites could be associated with the imbalance of neurotransmitters, mitochondrial dysfunction, and cell loss observed during both epileptogenesis and epilepsy. With these findings, we provided an overview of hippocampal metabolic profiles during different stages of epileptogenesis that could help investigate pathways and respective metabolites as predictive tools in epilepsy.\n                  <\/jats:p>","DOI":"10.1002\/bmc.5820","type":"journal-article","created":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T21:18:31Z","timestamp":1703798311000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Hippocampal metabolic profile during epileptogenesis in the pilocarpine model of epilepsy"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9848-9814","authenticated-orcid":false,"given":"Let\u00edcia","family":"Meier","sequence":"first","affiliation":[{"name":"Biosciences and Mass Spectrometry Laboratory, Department of Pharmacy Universidade Federal do Paran\u00e1  Curitiba PR Brazil"},{"name":"Graduate Program in Pharmaceutical Science 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Barbieri","family":"Caus","sequence":"additional","affiliation":[{"name":"Neurophysiology and Neurochemistry of Neuronal Excitability and Synaptic Plasticity Laboratory (NNNESP Lab.), Department of Biochemistry, ICBS Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayara Vendramin","family":"Pasquetti","sequence":"additional","affiliation":[{"name":"Neurophysiology and Neurochemistry of Neuronal Excitability and Synaptic Plasticity Laboratory (NNNESP Lab.), Department of Biochemistry, ICBS Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Elisa","family":"Calcagnotto","sequence":"additional","affiliation":[{"name":"Neurophysiology and Neurochemistry of Neuronal Excitability and Synaptic Plasticity Laboratory (NNNESP Lab.), Department of Biochemistry, ICBS Universidade Federal do Rio Grande do Sul  Porto Alegre RS 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